Monkey Anti-myelin basic protein antibody ELISA Kit | MBP elisa kit

Monkey Anti-myelin basic protein antibody ELISA Kit

Cat. Num. AAA746741

• Gene Names: Mbp; Mbps
• Reactivity: Monkey
• Synonyms: Anti-myelin basic protein antibody; Monkey Anti-myelin basic protein antibody ELISA Kit; MBP elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This Anti-MBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey Anti-MBP. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This Anti-MBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey Anti-MBP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MBP elisa kit

Principle of the Assay: Anti-MBP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with MBP antigen. Standards or samples are then added to the microtiter plate wells and anti-MBP if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of anti-MBP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated MBP antigen is added to each well to "sandwich" the anti-MBP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Anti-MBP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-MBP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MBP elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 70166270
• NCBI GeneID: 24547
• NCBI Accession #: NP_001020465.1
• NCBI GenBank Nucleotide #: NM_001025294.1
• Molecular Weight: 17,240 Da
• NCBI Official Full Name: myelin basic protein isoform 4
• NCBI Official Synonym Full Names: myelin basic protein
• NCBI Official Symbol: Mbp
• NCBI Official Synonym Symbols: Mbps
• NCBI Protein Information: Golli-Mbp; myelin basic protein; MBP S; myelin basic protein S; myelin basic protein Golli-mbp; Golli-Mbp; myelin basic protein; myelin basic protein S
• UniProt Protein Name: Myelin basic protein
• Protein Family: Myelin basic protein
• UniProt Gene Name: Mbp
• UniProt Synonym Gene Names: MBP
• UniProt Entry Name: MBP_RAT

NCBI Description

The protein encoded by the classic Mbp gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, Mbp-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long Mbp gene (otherwise called "Golli-Mbp") that contains 3 additional exons located upstream of the classic Mbp exons. Alternative splicing from the Golli and the Mbp transcription start sites gives rise to 2 sets of Mbp-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-Mbp, spliced in-frame to 1 or more Mbp exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to Mbp aa sequence. The second family of transcripts contain only Mbp exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the Mbp transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. Mutation of the Mbp gene is associated with the 'shiverer' and 'myelin deficient' phenotypes in mouse. [provided by RefSeq, Jul 2008]

Uniprot Description

MBP: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. Homodimer. Isoform 3 exists as a homodimer. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. Belongs to the myelin basic protein family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Cellular Component: compact myelin; internode region of axon; cell projection; cell soma; plasma membrane; myelin sheath

Molecular Function: protein binding; protease binding; structural constituent of myelin sheath

Biological Process: myelination; response to mercury ion; substantia nigra development; response to toxin; negative regulation of axonogenesis; response to progesterone stimulus; aging