Human methyl-CpG binding domain protein 1 ELISA Kit | MBD1 elisa kit

Human Methyl-CpG-binding domain protein 1, MBD1 ELISA Kit

Cat. Num. AAA9341675

• Gene Names: MBD1; RFT; PCM1; CXXC3
• Reactivity: Human
• Synonyms: methyl-CpG binding domain protein 1; Human Methyl-CpG-binding domain protein 1; MBD1 ELISA Kit; Human Methyl-CpG-binding domain protein 1 (MBD1) ELISA kit; CXXC3; PCM1; RFT; OTTHUMP00000163504; OTTHUMP00000163506; OTTHUMP00000163507; methyl-CpG binding domain protein 1 isoform PCM1; the regulator of fibroblast growth factor 2 (FGF-2) transcription; MBD1 elisa kit

• Reactivity: Human

• Preparation and Storage: Store all reagents at 2-8 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 323462147
• NCBI GeneID: 4152
• NCBI Accession #: NP_001191065.1
• NCBI GenBank Nucleotide #: NM_001204136.1
• UniProt Accession #: Q9UIS9; O15248; O95241; Q7Z7B5; Q8N4W4; Q9UNZ6; A4UTZ0; B4DXJ5; E9PEC5; K7ELI2; K7EQZ4; K7ESN0
• Molecular Weight: 66,607 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 1 isoform 6
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 1
• NCBI Official Symbol: MBD1
• NCBI Official Synonym Symbols: RFT; PCM1; CXXC3
• NCBI Protein Information: methyl-CpG-binding domain protein 1; CXXC-type zinc finger protein 3; protein containing methyl-CpG-binding domain 1; methyl-CpG binding domain protein 1 isoform PCM1; the regulator of fibroblast growth factor 2 (FGF-2) transcription
• UniProt Protein Name: Methyl-CpG-binding domain protein 1
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD1
• UniProt Synonym Gene Names: CXXC3; PCM1
• UniProt Entry Name: MBD1_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

MBD1: Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 18q21

Cellular Component: heterochromatin; nuclear matrix; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; DNA binding; methyl-CpG binding; zinc ion binding; transcription factor activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent