Human S-adenosylmethionine synthase isoform type-1 ELISA Kit | MAT1A elisa kit

Human S-adenosylmethionine synthase isoform type-1 ELISA Kit

Cat. Num. AAA763437

• Gene Names: MAT1A; MAT; SAMS; MATA1; SAMS1
• Reactivity: Human
• Synonyms: S-adenosylmethionine synthase isoform type-1; Human S-adenosylmethionine synthase isoform type-1 ELISA Kit; MAT1A/AMS1/MATA1/AdoMet synthase 1/S-adenosylmethionine synthase isoform type-1/Methionine adenosyltransferase 1; MAT1A elisa kit

• Reactivity: Human
• Sequence Length: 395

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MAT1A elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MAT1A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MAT1A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MAT1A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MAT1A can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4557737
• NCBI GeneID: 4143
• NCBI Accession #: NP_000420.1
• NCBI GenBank Nucleotide #: NM_000429.2
• Molecular Weight: 43,648 Da
• NCBI Official Full Name: S-adenosylmethionine synthase isoform type-1
• NCBI Official Synonym Full Names: methionine adenosyltransferase 1A
• NCBI Official Symbol: MAT1A
• NCBI Official Synonym Symbols: MAT; SAMS; MATA1; SAMS1
• NCBI Protein Information: S-adenosylmethionine synthase isoform type-1
• UniProt Protein Name: S-adenosylmethionine synthase isoform type-1
• Protein Family: S-adenosylmethionine synthase
• UniProt Gene Name: MAT1A
• UniProt Synonym Gene Names: AMS1; MATA1; AdoMet synthase 1; MAT 1; MAT-I/III
• UniProt Entry Name: METK1_HUMAN

NCBI Description

This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

MAT1A: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. Defects in MAT1A are the cause of methionine adenosyltransferase deficiency (MATD); also called MAT I/III deficiency. MATD is an inborn error of metabolism resulting in isolated hypermethioninemia. Most patients have no clinical abnormalities, although some neurologic symptoms may be present in rare cases with severe loss of methionine adenosyltransferase activity. Belongs to the AdoMet synthase family.

Protein type: Other Amino Acids Metabolism - selenoamino acid; EC 2.5.1.6; Amino Acid Metabolism - cysteine and methionine; Transferase

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: cytosol

Molecular Function: ATP binding; metal ion binding; methionine adenosyltransferase activity

Biological Process: amino acid metabolic process; methylation; one-carbon compound metabolic process; S-adenosylmethionine biosynthetic process; selenium metabolic process; sulfur amino acid metabolic process; xenobiotic metabolic process

Disease: Methionine Adenosyltransferase Deficiency