JIP1 Blocking Peptide | MAPK8IP1 blocking peptide

JIP1 Antibody (C-term) Blocking Peptide

• Gene Names: MAPK8IP1; IB1; JIP1; JIP-1; PRKM8IP
• Synonyms: JIP1; JIP1 Antibody (C-term) Blocking Peptide; C-Jun-amino-terminal kinase-interacting protein 1; JIP-1; JNK-interacting protein 1; Islet-brain 1; IB-1; JNK MAP kinase scaffold protein 1; Mitogen-activated protein kinase 8-interacting protein 1; MAPK8IP1; IB1; PRKM8IP; MAPK8IP1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human JIP1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 711

• Cellular Location: Cytoplasm. Cytoplasm, perinuclear region. Nucleus Endoplasmic reticulum membrane. Mitochondrion membrane Note: Accumulates in cell surface projections. Under certain stress conditions, translocates to the perinuclear region of neurons. In insulin-secreting cells, detected in both the cytoplasm and nucleus (By similarity).
• Tissue Location: Highly expressed in brain. Expressed in neurons, localizing to neurite tips in differentiating cells. Also expressed in the pancreas, testis and prostate. Low levels in heart, ovary and small intestine. Decreased levels in pancreatic beta cells sensitize cells to IL-1-beta-induced apoptosis
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MAPK8IP1 blocking peptide

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK- regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity). Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response.

NCBI and Uniprot Product Information

• NCBI GI #: 17433093
• NCBI GeneID: 9479
• UniProt Accession #: Q9UQF2; O43407; D3DQP4
• Molecular Weight: 77,524 Da
• NCBI Official Full Name: C-Jun-amino-terminal kinase-interacting protein 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8 interacting protein 1
• NCBI Official Symbol: MAPK8IP1
• NCBI Official Synonym Symbols: IB1; JIP1; JIP-1; PRKM8IP
• NCBI Protein Information: C-Jun-amino-terminal kinase-interacting protein 1
• UniProt Protein Name: C-Jun-amino-terminal kinase-interacting protein 1
• Protein Family: C-Jun-amino-terminal kinase-interacting protein
• UniProt Gene Name: MAPK8IP1
• UniProt Synonym Gene Names: IB1; JIP1; PRKM8IP; JIP-1; JNK-interacting protein 1; IB-1
• UniProt Entry Name: JIP1_HUMAN

NCBI Description

This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes. [provided by RefSeq, May 2011]

Uniprot Description

JIP1: a member of the JNK-interacting protein group of scaffold proteins that selectively mediates JNK signaling by aggregating specific components of the MAPK cascade. Required for JNK activation in response to excitotoxic stress. Causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and by inhibiting the JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly or indirectly regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interations with the JNK-signaling components and motor proteins.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cytoplasm

Molecular Function: kinesin binding; MAP-kinase scaffold activity; protein binding; protein kinase inhibitor activity

Biological Process: regulation of JNK cascade; vesicle-mediated transport

Disease: Diabetes Mellitus, Noninsulin-dependent