Mitogen-activated protein kinase kinase kinase 1 Recombinant Protein | M3K1 recombinant protein

Recombinant mouse Mitogen-activated protein kinase kinase kinase 1

Cat. Num. AAA9422036

• Gene Names: MAP3K1; MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Mitogen-activated protein kinase kinase kinase 1; Recombinant mouse Mitogen-activated protein kinase kinase kinase 1; MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1; M3K1 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Partial of the Full Length of 2-1493aa, 1216-1493aa

• Tag Info: His-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for M3K1 recombinant protein

Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 153945765
• NCBI GeneID: 4214
• NCBI Accession #: NP_005912.1
• NCBI GenBank Nucleotide #: NM_005921.1
• UniProt Accession #: Q13233
• Molecular Weight: 33.7kD
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 1
• NCBI Official Symbol: MAP3K1
• NCBI Official Synonym Symbols: MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 1
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MAP3K1
• UniProt Synonym Gene Names: MAPKKK1; MEKK; MEKK1; MEK kinase 1; MEKK 1

NCBI Description

The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012]

Uniprot Description

MEKK1: a protein kinase of the STE11 family. Phosphorylates and activates MKK4 and -7, which in turn activate JNK1, -2 and -3. Contains 1 RING-type zinc finger. The RING finger domain of MEKK1 exhibits E3 ubiquitin ligase activity toward c-Jun. c-Jun is downregulated in response to osmotic stress via ubiquitination-mediated degradation by the RING finger domain of MEKK1. Overexpressed/overactivated in multiple tumor types. The Mek1/2 inhibitor U0126 blocks export of influenza viral particles and has been suggested as an antiviral treatment. Single mutations at the same point of each gene also associated with cardiofaciocutaneous syndrome, (also linked with Braf and Kras mutations), displaying morphological, cardiac and mental defects. Inhibitors: U0126, CI-1040/PD184352, PD-0325901 (Phase I cancer), ARRY-142886 (Phase 1, cancer).

Protein type: EC 2.7.11.25; Kinase, protein; Ligase; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE11 family; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity

Biological Process: activation of protein kinase activity; MyD88-dependent toll-like receptor signaling pathway; regulation of apoptosis; regulation of mitotic cell cycle; stress-activated protein kinase signaling pathway

Disease: 46,xy Sex Reversal 6