MAP2K6 / Dual Specificity Mitogen-Activated Protein Kinase Kinase 6 Recombinant Protein | MAP2K6 recombinant protein

Human MAP2K6 / Dual Specificity Mitogen-Activated Protein Kinase Kinase 6 Recombinant Protein

• Gene Names: MAP2K6; MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; SAPKK-3
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: MAP2K6 / Dual Specificity Mitogen-Activated Protein Kinase Kinase 6; Human MAP2K6 / Dual Specificity Mitogen-Activated Protein Kinase Kinase 6 Recombinant Protein; Dual specificity mitogen-activated protein kinase kinase 6; MAP kinase kinase 6; MAPK/ERK kinase 6; MEK 6; Stress-activated protein kinase kinase 3; SAPK kinase 3; MEK6; MKK6; PRKMK6; SKK3; MAP2K6 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 334

• Applicable Applications for MAP2K6 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Dimer. Interacts with Yersinia yopJ. Interacts (via its D domain) with its substrates MAPK11, MAPK12, MAPK13 and MAPK14 (By similarity). Interacts (via its DVD domain) with MAP3Ks activators like MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK3, MAP3K4/MEKK4, MAP3K7/TAK1, MAP3K11/MLK3 and MAP3K17/TAOK2 (By similarity). Interacts with DCTN1. Interacts with EIF2AK2/PKR.
• Entry Function: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 14589900
• NCBI GeneID: 5608
• NCBI Accession #: NP_002749.2
• NCBI GenBank Nucleotide #: NP_002749.2
• UniProt Accession #: P52564
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 6 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 6
• NCBI Official Symbol: MAP2K6
• NCBI Official Synonym Symbols: MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; SAPKK-3
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 6
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 6
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K6
• UniProt Synonym Gene Names: MEK6; MKK6; PRKMK6; SKK3; MAP kinase kinase 6; MAPKK 6; MEK 6; SAPK kinase 3; SAPKK-3; SAPKK3
• UniProt Entry Name: MP2K6_HUMAN

NCBI Description

This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

MKK6: a dual-specificity protein kinase of the STE7 family. Activates p38 MAP kinase by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by cytokines and environmental stress in vivo. Three alternatively spliced isoforms have been reported.

Protein type: EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE7 family

Chromosomal Location of Human Ortholog: 17q24.3

Cellular Component: nucleoplasm; cytoskeleton; cytosol

Molecular Function: MAP kinase kinase activity; protein binding; protein-tyrosine kinase activity; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; DNA damage induced protein phosphorylation; activation of MAPK activity; positive regulation of apoptosis; apoptosis; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; toll-like receptor 10 signaling pathway; ovulation cycle process; toll-like receptor 5 signaling pathway; regulation of transcription, DNA-dependent; cell cycle arrest; toll-like receptor 4 signaling pathway; cardiac muscle contraction; response to drug; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; muscle cell differentiation; positive regulation of prostaglandin secretion; toll-like receptor signaling pathway; positive regulation of muscle cell differentiation; innate immune response; toll-like receptor 9 signaling pathway; positive regulation of nitric-oxide synthase biosynthetic process