Mitogen-Activated Protein Kinase Kinase 2 Recombinant Protein | MAP2K2 recombinant protein

Recombinant Human Mitogen-Activated Protein Kinase Kinase 2

Cat. Num. AAA145083

• Gene Names: MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Mitogen-Activated Protein Kinase Kinase 2; Recombinant Human Mitogen-Activated Protein Kinase Kinase 2; MAP2K2 Human; Mitogen-Activated Protein Kinase Kinase 2 Human Recombinant; Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2; MAP2K2; MEK2; MKK2; PRKMK2; MAPKK2; MAP2K2 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: MAP2K2 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl and 10% glycerol.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMLARRK PVLPALTINP TIAEGPSPTS EGASEANLVD LQKKLEELEL DEQQKKRLEA FLTQKAKVGE LKDDDFERIS ELGAGNGGVV TKVQHRPSGL IMARKLIHLE IKPAIRNQII RELQVLHECN SPYIVGFYGA FYSDGEISIC MEHMDGGSLD QVLKEAKRIP EEILGKVSIA VLRGLAYLRE KHQIMHRDVK PSNILVNSRG EIKLCDFGVS GQLIDSMANS FVGTRSYMAP ERLQGTHYSV QSDIWSMGLS LVELAVGRYP IPPPDAKELE AIFGRPVVDG EEGEPHSISP RPRPPGRPVS GHGMDSRPAM AIFELLDYIV NEPPPKLPNG VFTPDFQEFV NKCLIKNPAE RADLKMLTNH TFIKRSEVEE VDFAGWLCKT LRLNQPGTPT RTAV.
• Sequence Length: 400

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for MAP2K2 recombinant protein

Description: MAP2K2 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 424 amino acids (1-400 a.a.) and having a molecular mass of 46.9kDa.MAP2K2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) is a dual specificity protein kinase, which is a member of the STE grouping of kinases and the MAP kinase kinase family. MAP2K2 is activated by a wide range of growth factors and cytokines and also by membrane depolarization and calcium influx. The MAP2K2 kinase is known for its vital role in mitogen growth factor signal transduction. MAP2K2 phosphorylates and consequently activates MAPK1/ERK2 and MAPK2/ERK3. The activation of the MAP2K2 kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of the MAP2K2 kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. MAP2K2 gene mutations cause cardiofaciocutaneous syndrome (CFC syndrome).

Product Categories/Family for MAP2K2 recombinant protein

Protein Kinases; Enzymes; Mitogen-Activated Protein Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 13489054
• NCBI GeneID: 5605
• NCBI Accession #: NP_109587.1
• NCBI GenBank Nucleotide #: NM_030662.3
• UniProt Accession #: P36507
• Molecular Weight: 44,424 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 2
• NCBI Official Symbol: MAP2K2
• NCBI Official Synonym Symbols: CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MAP kinase kinase 2; MAPK/ERK kinase 2; mitogen-activated protein kinase kinase 2, p45
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 2
• Protein Family: Mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K2
• UniProt Synonym Gene Names: MEK2; MKK2; PRKMK2; MAP kinase kinase 2; MAPKK 2; MEK 2
• UniProt Entry Name: MP2K2_HUMAN

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MEK2: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf and Mos kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK2 and ERK3. A component of MAP kinase signal transduction pathways involved in mitogen growth factor signal transduction.

Protein type: Protein kinase, STE; EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Kinase, protein; STE group; STE7 family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: peroxisomal membrane; Golgi apparatus; microtubule; internal side of plasma membrane; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; extracellular region; intercellular junction; cell cortex; cytosol; perinuclear region of cytoplasm; late endosome; cytoplasm; nucleus

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; protein complex scaffold; ATP binding; PDZ domain binding

Biological Process: epidermal growth factor receptor signaling pathway; axon guidance; fibroblast growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; activation of MAPKK activity; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; MAPKKK cascade; pathogenesis; toll-like receptor 3 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of stress-activated MAPK cascade; small GTPase mediated signal transduction; Ras protein signal transduction; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; vascular endothelial growth factor receptor signaling pathway; toll-like receptor 4 signaling pathway

Disease: Cardiofaciocutaneous Syndrome 4