MAOA recombinant protein

Recombinant MAOA protein

Cat. Num. AAA388212

• Gene Names: MAOA; MAO-A
• Purity: The recombinant protein is >80% pure by SDS-PAGE.
• Synonyms: MAOA; Recombinant MAOA protein; Monoamine Oxidase A; MAO-A; MAOA recombinant protein

• Host: Baculovirus
• Purity/Purification: The recombinant protein is >80% pure by SDS-PAGE.
• Form/Format: Full length recombinant MAOA protein was expressed in Sf9 cells and is supplied in 25mM Hepes pH7.5, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2mM TCEP, 0.2mg/ml 3× DYKDDDDK peptide.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: Recombinant MAOA is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Monoamine Oxidase Assay: 40uM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Detection Reagent for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-Glo Assay.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant MAOA protein gel. MAOA protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)

Testing Data

(MAO-GloTM Assay for MAOA activity 40 uM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Detection Reagent for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-Glo Assay.)

Related Product Information for MAOA recombinant protein

Short Description: Recombinant MAOA (accession number NP_000231.1) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK-Tag with an observed molecular weight of 60.7 kDa. The recombinant protein is >80% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: MAOA (Monoamine Oxidase A) is one of two neighboring mitochondrial enzyme family members which catalyze the oxidative deamination of amines, such as dopamine, norepinephrine, and serotonin. The other member is MAOB. Mutation of MAOA results in Brunner syndrome and maoa-related behavior disorders. MAOA has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.

Product Categories/Family for MAOA recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4557735
• NCBI GeneID: 4128
• NCBI Accession #: NP_000231.1
• NCBI GenBank Nucleotide #: NM_000240.3
• UniProt Accession #: P21397; Q16426; B4DF46
• Molecular Weight: 44,848 Da
• NCBI Official Full Name: amine oxidase
• NCBI Official Synonym Full Names: monoamine oxidase A
• NCBI Official Symbol: MAOA
• NCBI Official Synonym Symbols: MAO-A
• NCBI Protein Information: amine oxidase [flavin-containing] A
• UniProt Protein Name: Amine oxidase [flavin-containing] A
• Protein Family: Amine oxidase
• UniProt Gene Name: MAOA
• UniProt Synonym Gene Names: MAO-A
• UniProt Entry Name: AOFA_HUMAN

Uniprot Description

MAOA: Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine. Defects in MAOA are the cause of Brunner syndrome (BRUNS). Brunner syndrome is a form of X-linked non- dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior. Belongs to the flavin monoamine oxidase family.

Protein type: EC 1.4.3.4; Membrane protein, integral; Amino Acid Metabolism - tryptophan; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - histidine; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - glycine, serine and threonine; Oxidoreductase; Amino Acid Metabolism - tyrosine

Chromosomal Location of Human Ortholog: Xp11.3

Cellular Component: mitochondrial outer membrane; mitochondrion; integral to membrane

Molecular Function: amine oxidase activity

Biological Process: synaptic transmission; biogenic amine metabolic process; neurotransmitter catabolic process; dopamine catabolic process; xenobiotic metabolic process; neurotransmitter secretion; neurotransmitter biosynthetic process

Disease: Brunner Syndrome