Human Glycoprotein endo-alpha-1,2-mannosidase ELISA Kit | MANEA elisa kit
Human Glycoprotein endo-alpha-1,2-mannosidase (MANEA) ELISA Kit
Gene Names
MANEA; ENDO; hEndo
Reactivity
Human
Synonyms
Glycoprotein endo-alpha-1; 2-mannosidase; Human Glycoprotein endo-alpha-1; 2-mannosidase (MANEA) ELISA Kit; MANEA elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human MANEA. No significant cross-reactivity or interference between Human MANEA and analogues was observed.
Sequence Length
143
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for MANEA elisa kit
N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase, such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans. The deduced 462-amino acid protein contains a putative type II transmembrane domain near the N terminus and shares about 77.9% identity with rat liver Manea.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MANEA in samples. An antibody specific for MANEA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMANEA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MANEA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MANEA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MANEA in samples. An antibody specific for MANEA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMANEA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MANEA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MANEA bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,671 Da
NCBI Official Full Name
glycoprotein endo-alpha-1,2-mannosidase
NCBI Official Synonym Full Names
mannosidase endo-alpha
NCBI Official Symbol
MANEA
NCBI Official Synonym Symbols
ENDO; hEndo
NCBI Protein Information
glycoprotein endo-alpha-1,2-mannosidase
UniProt Protein Name
Glycoprotein endo-alpha-1,2-mannosidase
Protein Family
UniProt Gene Name
MANEA
UniProt Synonym Gene Names
Endo-alpha mannosidase; Endomannosidase; hEndo
NCBI Description
N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM, Sep 2008]
Uniprot Description
MANEA: Belongs to the glycosyl hydrolase 99 family
Protein type: EC 3.2.1.130; Hydrolase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 6q16.1
Cellular Component: Golgi apparatus; Golgi membrane; integral component of membrane
Molecular Function: glycoprotein endo-alpha-1,2-mannosidase activity
Research Articles on MANEA
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Product Notes
The Human MANEA manea (Catalog #AAA282245) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282245 ELISA Kit recognizes Human MANEA. It is sometimes possible for the material contained within the vial of "Glycoprotein endo-alpha-1,2-mannosidase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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