Rabbit Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA ELISA Kit | MAN1A1 elisa kit
Rabbit Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) ELISA Kit
Reactivity
Rabbit
Synonyms
Mannosyl-oligosaccharide 1; 2-alpha-mannosidase IA; Rabbit Mannosyl-oligosaccharide 1; 2-alpha-mannosidase IA (MAN1A1) ELISA Kit; HUMM3; HUMM9; MAN9; Man9-mannosidase|alpha-1; 2-mannosidase IA|mannosyl-oligosaccharide 1; MAN1A1 elisa kit
Reactivity
Rabbit
Specificity
This assay has high sensitivity and excellent specificity for detection of Rabbit MAN1A1. No significant cross-reactivity or interference between Rabbit MAN1A1 and analogues was observed.
Sequence Length
653
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for MAN1A1 elisa kit
MAN1A1 encodes a class I mammalian Golgi 1,2-mannosidase which is a type II transmembrane protein. This protein catalyzes the removal of 3 distinct mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides and belongs to family 47 of glycosyl hydrolases. Using an oligonucleotide probe derived from a pig liver Man(9)-mannosidase-specific cDNA template, Bause et al. (1993) isolated Man(9)-mannosidase from a human kidney cDNA library. The full-length cDNA predicted a 625-amino acid protein with a calculated molecular mass of 71 kD. Man(9)-mannosidase is a type II transmembrane protein with a short cytoplasmic polypeptide tail, a single transmembrane domain acting as a noncleavable signal sequence, a large luminal catalytic domain, and 1 potential N-glycosylation sites.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MAN1A1 in samples. An antibody specific for MAN1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAN1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAN1A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAN1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MAN1A1 in samples. An antibody specific for MAN1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAN1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAN1A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAN1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
53,427 Da
NCBI Official Full Name
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
NCBI Official Symbol
MAN1A1
NCBI Protein Information
mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
UniProt Protein Name
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
Protein Family
UniProt Gene Name
MAN1A1
UniProt Synonym Gene Names
Alpha-1,2-mannosidase IA
Uniprot Description
Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.
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Product Notes
The Rabbit MAN1A1 man1a1 (Catalog #AAA284801) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA284801 ELISA Kit recognizes Rabbit MAN1A1. It is sometimes possible for the material contained within the vial of "Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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