Typical Standard Curve/Testing Data
Mammalian Homocysteine Assay Kit
Homocysteine Assay Kit (Fluorometric)
Reactivity
Mammalian
Synonyms
Homocysteine; Homocysteine Assay Kit (Fluorometric); Homocysteine assay kit
Reactivity
Mammalian
Application Notes
Applications- Estimation of Homocysteine in various biological samples. ; Detection limit/sensitivity: Can detect as little as 5 uM of Homocysteine
Samples
Biological fluids (serum/plasma)
Preparation and Storage
Store at -20 degree C for 12 months.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for Homocysteine assay kit
Background/Introduction: Homocysteine is a non-proteogenic amino acid synthesized intracellularly by removal of the N-methyl group from the essential amino acid methionine. Homocysteine is exported from cells into the blood, where it exists mainly as an oxidized disulfide species, either as a dimer or bound to cysteine residues of serum proteins. The reduced form of homocysteine ('free' homocysteine) can be metabolized into cysteine via the transsulfuration pathway; however, it can also undergo intramolecular cyclization, forming the highly reactive pro-oxidant homocysteine thiolactone. Subsequent N-homocysteinylation of protein lysine residues by the reactive thiolactone disrupts protein conformation, leading to formation of cytotoxic protein aggregates. Homocysteinylated proteins may also act as autoantigens, triggering arterial inflammation and atherosclerosis. Elevated plasma homocysteine concentration is a clinical biomarker for increased risk of cardiovascular disease, ischemic stroke and myocardial infarction. Severely elevated homocysteine levels (hyperhomocysteinemia) are correlated with a 4-fold increase in mortality due to heart attack and a 16-fold increase in the likelihood of recurrent stroke. Homocysteine Assay Kit allows for quantification of total homocysteine in biological fluids such as plasma and serum. The assay is based on the reduction of homocysteine disulfides to free homocysteine, which is cleaved by a homocysteine-selective enzyme, generating an intermediate product. The intermediate reacts with a probe solution to form a stable fluorophore that emits in the far-red spectrum (Ex/Em = 658/708 nm). The assay is not affected by physiological concentrations of other biological thiols (such as cysteine, methionine and glutathione), is high-throughput adaptable and can detect as low as 5 uM homocysteine.
Product Categories/Family for Homocysteine assay kit
Similar Products
Product Notes
The Mammalian Homocysteine (Catalog #AAA846809) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA846809 Assay Kit recognizes Mammalian Homocysteine. Applications- Estimation of Homocysteine in various biological samples. ; Detection limit/sensitivity: Can detect as little as 5 uM of Homocysteine. Researchers should empirically determine the suitability of the Homocysteine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Homocysteine, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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