Chicken Magnesium transporter protein 1 ELISA Kit | MAGT1 elisa kit

Chicken Magnesium transporter protein 1 (MAGT1) ELISA Kit

Cat. Num. AAA284440

• Reactivity: Chicken
• Synonyms: Magnesium transporter protein 1; Chicken Magnesium transporter protein 1 (MAGT1) ELISA Kit; RP11-217H1.1; DKFZp564K142; FLJ14726; IAP; MGC64926; OST3B; PRO0756; bA217H1.1; implantation-associated protein; MAGT1 elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chicken MAGT1. No significant cross-reactivity or interference between Chicken MAGT1 and analogues was observed.
• Sequence Length: 367

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MAGT1 elisa kit

MAGT1 encodes a magnesium cation transporter protein that localizes to the cell membrane. This protein also associates with N-oligosaccharyl transferase and therefore may have a role in N-glycosylation. Mutations in this gene cause mental retardation X-linked type 95 (MRX95). This gene may have multiple in-frame translation initiation sites, one of which would encode a shorter protein with an N-terminus containing a signal peptide at amino acids 1-29.The deduced 335-amino acid human protein has a calculated molecular mass of 38 kD. MAGT1 contains 5 putative transmembrane domains, the first of which is likely cleaved. It has an N-glycosylation site in its first extracellular loop and several putative phosphorylation sites in its N-terminal region. Northern blot analysis detected variable Magt4 expression in all mouse tissues examined.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MAGT1 in samples. An antibody specific for MAGT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAGT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAGT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAGT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 57530159
• NCBI GeneID: 422332
• NCBI Accession #: NP_001006435.1
• NCBI GenBank Nucleotide #: NM_001006435.1
• UniProt Accession #: Q5ZJ06
• Molecular Weight: 36,762 Da
• NCBI Official Full Name: magnesium transporter protein 1
• NCBI Official Synonym Full Names: magnesium transporter 1
• NCBI Official Symbol: MAGT1
• NCBI Protein Information: magnesium transporter protein 1
• UniProt Protein Name: Magnesium transporter protein 1
• Protein Family: Magnesium transporter protein
• UniProt Gene Name: MAGT1
• UniProt Synonym Gene Names: MagT1

Uniprot Description

Acts as accessory component of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Involved in N-glycosylation of STT3B-dependent substrates. Specifically required for the glycosylation of a subset of acceptor sites that are near cysteine residues. In its oxidized form proposed to form transient mixed disulfides with a glycoprotein substrate to facilitate access of STT3B to the unmodified acceptor site. Has also oxidoreductase-independent functions in the STT3B-containing OST complex possibly involving substrate recognition.