Rabbit anti-Human NPPC Polyclonal Antibody | anti-NPPC antibody

NPPC, CT (NPPC, CNP2, C-type natriuretic peptide, CNP-22, CNP-29, CNP-53) (Azide free) (HRP)

Cat. Num. AAA6326307

• Gene Names: NPPC; CNP; CNP2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NPPC; Polyclonal Antibody; CT (NPPC; CNP2; C-type natriuretic peptide; CNP-22; CNP-29; CNP-53) (Azide free) (HRP); CNP-53; anti-NPPC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NPPC antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NPPC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-109 amino acids from the C-terminal region of human NPPC.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NPPC Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NPPC antibody detected the NPPC protein (arrow).)

Immunohistochemistry (IHC)

(NPPC Antibody (C-term) (AP14031b)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NPPC Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-NPPC antibody

Antibodies; Proteins

References

Lippert, S.K., et al. J. Immunol. Methods 362 (1-2), 32-37 (2010): Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Saus, E., et al. J Psychiatr Res 44(14):971-978(2010). Wang, Y., et al. J. Cardiovasc. Pharmacol. 55(1):62-66(2010). Cammarata, P.R., et al. Mol. Vis. 16, 630-638 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 13249346
• NCBI GeneID: 4880
• NCBI Accession #: NP_077720.1
• NCBI GenBank Nucleotide #: NM_024409.2
• UniProt Accession #: P23582; Q4ZG41
• Molecular Weight: 13,246 Da
• NCBI Official Full Name: C-type natriuretic peptide
• NCBI Official Synonym Full Names: natriuretic peptide C
• NCBI Official Symbol: NPPC
• NCBI Official Synonym Symbols: CNP; CNP2
• NCBI Protein Information: C-type natriuretic peptide; natriuretic peptide precursor C
• UniProt Protein Name: C-type natriuretic peptide
• Protein Family: C-type natriuretic peptide
• UniProt Gene Name: NPPC
• UniProt Synonym Gene Names: CNP2
• UniProt Entry Name: ANFC_HUMAN

NCBI Description

The protein encoded by this gene is proteolytically processed to form a secreted hormone of the natriuretic peptide family. The encoded hormone regulates the growth and differentiation of cartilaginous growth plate chondrocytes and may also be vasoactive and natriuretic. [provided by RefSeq, Sep 2011]

Uniprot Description

NPPC: Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.

Protein type: Secreted, signal peptide; Secreted; Hormone

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: extracellular space; secretory granule

Molecular Function: protein homodimerization activity; peptide hormone receptor binding; hormone activity; receptor binding

Biological Process: response to drug; cGMP biosynthetic process; ossification; regulation of smooth muscle cell proliferation; regulation of cAMP metabolic process; regulation of multicellular organism growth; negative regulation of meiotic cell cycle; positive regulation of vasodilation; negative regulation of DNA metabolic process; post-embryonic development; negative regulation of cell proliferation; positive regulation of osteoblast differentiation; response to ethanol; positive regulation of cGMP biosynthetic process; receptor guanylyl cyclase signaling pathway; response to hypoxia; regulation of cGMP metabolic process; regulation of blood vessel size