MAGOH recombinant protein

MAGOH, 1-146aa, Human, His tag, E Coli

Cat. Num. AAA203529

• Gene Names: MAGOH; MAGOH1; MAGOHA
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: MAGOH; 1-146aa; Human; His tag; E Coli; Protein mago nashi homolog; MAGOH1; MAGOHA; Protein mago nashi homolog Mago nashi homolog proliferation associated (Drosophila); Mago nashi protein homolog.; MAGOH recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 0.1M NaCl, 20% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA YVHKSVMEEL KRIIDDSEIT KEDDALWPPP DRVGRQELEI VIGDEHISFT TSKIGSLIDV NQSKDPEGLR VFYYLVQDLK CLVFSLIGLH FKIKPILEHH HHHH
• Sequence Length: 146

• Applicable Applications for MAGOH recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for MAGOH recombinant protein

MAGOH, the human homolog of Drosophila mago nashi, is required for embryo development. MAGOH is ubiquitously expressed in adult tissues. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. Recombinant human MAGOH protein, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for MAGOH recombinant protein

Transcription & Translation

References

Gehring NH., et al. (2009) Cell. 137 (3):536-48. ; Chamieh H., et al. (2008) Nat Struct Mol Biol. 15 (1):85-93.;

NCBI and Uniprot Product Information

• NCBI GI #: 4505087
• NCBI GeneID: 4116
• NCBI Accession #: NP_002361
• NCBI GenBank Nucleotide #: NM_002370.3
• UniProt Accession #: P61326; O35169; P50606; Q5SW69; B1ARP8; B2R5A2
• Molecular Weight: 18.2 kDa (154aa), confirmed by MALDI-TOF
• NCBI Official Full Name: protein mago nashi homolog
• NCBI Official Synonym Full Names: mago homolog, exon junction complex core component
• NCBI Official Symbol: MAGOH
• NCBI Official Synonym Symbols: MAGOH1; MAGOHA
• NCBI Protein Information: protein mago nashi homolog
• UniProt Protein Name: Protein mago nashi homolog
• Protein Family: Protein mago nashi
• UniProt Gene Name: MAGOH
• UniProt Synonym Gene Names: MAGOHA

NCBI Description

Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. [provided by RefSeq, Jul 2008]

Uniprot Description

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.