MAFF recombinant protein

Recombinant Human MAFF

Cat. Num. AAA7614539

• Gene Names: MAFF; U-MAF; hMafF
• Applications: Western Blot, ELISA
• Purity: Greater than 90% by SDS-PAGE gel analyses
• Synonyms: MAFF; Recombinant Human MAFF; Avian musculoaponeurotic fibrosarcoma virus (v maf) AS42 oncogene; protein F; CTA 447C4.1; hMafF; MAFF_HUMAN; OTTHUMP00000199299; Transcription factor MafF; U MAF; U-Maf; V maf avian musculoaponeurotic fibrosarcoma oncogene family; V maf musculoaponeurotic fibrosarcoma oncogene homolog F; V maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian); MAFF recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% by SDS-PAGE gel analyses
• Form/Format: Lyophilized from a 0.2um filtered solution in PBS with 5% trehalose, pH7.4
• Sequence Positions: 1-164

• Applicable Applications for MAFF recombinant protein: Western Blot (WB), ELISA (EIA)
• Species: Human
• Tag: N-terminal His-IF2DI Tag
• Reconstitution: Centrifuge the vial at 10,000rpm for 1 minute, reconstitute at 200ug/ml in sterile distilled water
• Preparation and Storage: Store at -20 degree C for 12 months as lyophilized; 2-8 degree C for 1 month under sterile conditions after reconstitution

NCBI and Uniprot Product Information

• NCBI GI #: 239048350
• NCBI GeneID: 23764
• NCBI Accession #: NP_001155044.1
• NCBI GenBank Nucleotide #: NM_001161572.1
• UniProt Accession #: Q9ULX9; Q9Y525; B4DV49
• Molecular Weight: 17,760 Da
• NCBI Official Full Name: transcription factor MafF isoform a
• NCBI Official Synonym Full Names: v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)
• NCBI Official Symbol: MAFF
• NCBI Official Synonym Symbols: U-MAF; hMafF
• NCBI Protein Information: transcription factor MafF; v-maf avian musculoaponeurotic fibrosarcoma oncogene family protein F
• UniProt Protein Name: Transcription factor MafF
• Protein Family: MaFF-interacting protein
• UniProt Gene Name: MAFF
• UniProt Entry Name: MAFF_HUMAN

NCBI Description

The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

MAFF: Interacts with the upstream promoter region of the oxytocin receptor gene. May be a transcriptional enhancer in the up-regulation of the oxytocin receptor gene at parturition. Since it lacks a putative transactivation domain, it may behave as a transcriptional repressor when it dimerize among himself. May also serve as a transcriptional activator by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. May be involved in the cellular stress response. Belongs to the bZIP family. Maf subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; in utero embryonic development; regulation of epidermal cell differentiation; parturition; blood coagulation