Simian Macrophage Colony-Stimulating Factor ELISA Kit | M-CSF elisa kit

Simian Macrophage Colony-Stimulating Factor (M-CSF) ELISA Kit

Cat. Num. AAA281081

• Gene Names: CSF2; CSF; GMCSF
• Reactivity: Simian
• Synonyms: Macrophage Colony-Stimulating Factor; Simian Macrophage Colony-Stimulating Factor (M-CSF) ELISA Kit; M-CSF elisa kit

• Reactivity: Simian
• Specificity: This assay has high sensitivity and excellent specificity for detection of SimianMCSF. No significant cross-reactivity or interference between SimianMCSF and analogues was observed.
• Sequence Length: 553

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for M-CSF elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MCSF in samples. An antibody specific for MCSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCSF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCSF bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 27437030
• NCBI GeneID: 1437
• NCBI Accession #: NP_000749.2
• NCBI GenBank Nucleotide #: NM_000758.3
• UniProt Accession #: P04141; Q14CE8; Q2VPI8; Q8NFI6
• Molecular Weight: 16,295 Da
• NCBI Official Full Name: granulocyte-macrophage colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 2
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: CSF; GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• Protein Family: Macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

Uniprot Description

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular region; extracellular space; intracellular membrane-bound organelle

Molecular Function: cytokine activity; granulocyte macrophage colony-stimulating factor receptor binding; growth factor activity; protein binding; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: embryonic placenta development; epithelial fluid transport; histamine secretion; immune response; macrophage activation; MAPK cascade; monocyte differentiation; myeloid dendritic cell differentiation; negative regulation of cytolysis; neutrophil differentiation; peptidyl-tyrosine phosphorylation; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of GTPase activity; positive regulation of interleukin-23 production; positive regulation of tyrosine phosphorylation of STAT protein; regulation of circadian sleep/wake cycle, sleep; regulation of myeloid cell differentiation; response to silicon dioxide