Human Macrophage Colony-Stimulating Factor ELISA Kit | M-CSF elisa kit

Human Macrophage Colony-Stimulating Factor ELISA Kit

Cat. Num. AAA16322

• Gene Names: CSF1; MCSF; CSF-1
• Reactivity: Human
• Synonyms: Macrophage Colony-Stimulating Factor; Human Macrophage Colony-Stimulating Factor ELISA Kit; M-CSF elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human CSF-1 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-1000pg/ml.
• Sensitivity: 5.0pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated CSF-1 concentrations in Human serum, plasma and other body fluids. Using Purified Human CSF-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CSF-1 and CSF-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CSF-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 181135
• NCBI GeneID: 1435
• UniProt Accession #: P09603; Q13130; Q14086; Q14806; Q5VVF3; Q5VVF4; Q9UQR8; A8K6J5
• Molecular Weight: 60,179 Da
• NCBI Official Full Name: colony-stimulating factor (CSF-1)
• NCBI Official Synonym Full Names: colony stimulating factor 1 (macrophage)
• NCBI Official Symbol: CSF1
• NCBI Official Synonym Symbols: MCSF; CSF-1
• NCBI Protein Information: macrophage colony-stimulating factor 1; lanimostim
• UniProt Protein Name: Macrophage colony-stimulating factor 1
• Protein Family: Macrophage colony-stimulating factor
• UniProt Gene Name: CSF1
• UniProt Synonym Gene Names: CSF-1; M-CSF; MCSF
• UniProt Entry Name: CSF1_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

Uniprot Description

M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; integral to membrane; plasma membrane; receptor complex

Molecular Function: protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding

Biological Process: positive regulation of monocyte differentiation; macrophage differentiation; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; osteoclast differentiation; positive regulation of multicellular organism growth; odontogenesis; monocyte activation; positive regulation of cell proliferation; hemopoiesis; inflammatory response; positive regulation of cell-matrix adhesion; positive regulation of macrophage differentiation; cell differentiation; ossification; positive regulation of odontogenesis of dentine-containing teeth; reproductive developmental process; cell proliferation; positive regulation of mononuclear cell proliferation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; innate immune response; positive regulation of Ras protein signal transduction; regulation of ossification; positive regulation of cell migration