Porcine Lysozyme ELISA Kit | LZM elisa kit

Porcine Lysozyme ELISA Kit

Cat. Num. AAA764854

• Gene Names: LYZ; LZM; LYZF1
• Reactivity: Porcine
• Synonyms: Lysozyme; Porcine Lysozyme ELISA Kit; LZM elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of LZM. No significant cross-reactivity or interference between LZM and analogues was observed.
• Sequence Length: 148

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LZM elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the HRP conjugated antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 307142
• NCBI GeneID: 4069
• Molecular Weight: 16,537 Da
• NCBI Official Full Name: lysozyme (EC 3.2.1.17)
• NCBI Official Synonym Full Names: lysozyme
• NCBI Official Symbol: LYZ
• NCBI Official Synonym Symbols: LZM; LYZF1
• NCBI Protein Information: lysozyme C
• UniProt Protein Name: Lysozyme C
• Protein Family: Lysozyme
• UniProt Gene Name: LYZ
• UniProt Synonym Gene Names: LZM
• UniProt Entry Name: LYSC_HUMAN

NCBI Description

This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014]

Uniprot Description

LYZ: Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte- macrophage system and enhance the activity of immunoagents. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Belongs to the glycosyl hydrolase 22 family.

Protein type: EC 3.2.1.17; Vesicle; Endoplasmic reticulum; Secreted; Secreted, signal peptide; Hydrolase

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: extracellular region; extracellular space

Molecular Function: identical protein binding; lysozyme activity

Biological Process: cellular protein metabolic process; cytolysis; defense response to bacterium; inflammatory response; retinal homeostasis

Disease: Amyloidosis, Familial Visceral