SYF c-Src-Transformed + Pervanadate Lysate
SYF c-Src-Transformed + Pervanadate Lysate
Applications
Western Blot
Synonyms
SYF c-Src-Transformed + Pervanadate; SYF c-Src-Transformed + Pervanadate Lysate; SYF c-Src-Transformed + Pervanadate lysate
Form/Format
Supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% beta-mercaptoethanol).
Applicable Applications for SYF c-Src-Transformed + Pervanadate lysate
Western Blot (WB)
Application Notes
WB: 20 mul/lane
Lysate Preparation
c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion.
The mouse fibroblast cell line, SYF, is deficient for the c-Src family kinases, c-Src, Yes, and Fyn, while SYF + c-Src overexpress c-Src kinase. This cell line is useful for studies of c-Src mediated phosphorylation when treated with the tyrosine phosphatase inhibitor, pervanadate. Confluent cultures of SYF + c-Src cells were serum starved 2 hrs, and then either left untreated or treated with pervanadate (1 mM) for 30 minutes at 37 degree C. Cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.
The mouse fibroblast cell line, SYF, is deficient for the c-Src family kinases, c-Src, Yes, and Fyn, while SYF + c-Src overexpress c-Src kinase. This cell line is useful for studies of c-Src mediated phosphorylation when treated with the tyrosine phosphatase inhibitor, pervanadate. Confluent cultures of SYF + c-Src cells were serum starved 2 hrs, and then either left untreated or treated with pervanadate (1 mM) for 30 minutes at 37 degree C. Cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.
Preparation and Storage
Store at -20 degree C. Do not boil or dilute. Stable for 1 year.
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Product Notes
The SYF c-Src-Transformed + Pervanadate (Catalog #AAA474139) is a Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SYF c-Src-Transformed + Pervanadate can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 20 mul/lane. Researchers should empirically determine the suitability of the SYF c-Src-Transformed + Pervanadate for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SYF c-Src-Transformed + Pervanadate, Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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