Hamster Leukotriene C4 ELISA Kit | LTC4 elisa kit

Hamster Leukotriene C4 ELISA Kit

Cat. Num. AAA7338

• Reactivity: Hamster
• Synonyms: Leukotriene C4; Hamster Leukotriene C4 ELISA Kit; LTC4 elisa kit

• Reactivity: Hamster
• Sequence Length: 150

• Samples: Serum, Plasma, Cell Culture Supernatants, Tissue Homogenate, Urine and other Body Fluids
• Detection Range: 15.6 pg/ml ~ 500 pg/ml.
• Sensitivity: Typically less than 5.0 pg/ml.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Hamster LTC4 in the specimen, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the specimens and allow the operator to produce a standard curve of Optical Density versus Hamster LTC4 concentration. The concentration of Hamster LTC4 in the specimens is then determined by comparing the O.D. of the specimens to the standard curve.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 22035629
• NCBI GeneID: 4056
• NCBI Accession #: NP_665874.1
• NCBI GenBank Nucleotide #: NM_145867.1
• UniProt Accession #: Q16873; Q8N6P0; Q9UC73; Q9UD18
• Molecular Weight: 16,567 Da
• NCBI Official Full Name: leukotriene C4 synthase
• NCBI Official Synonym Full Names: leukotriene C4 synthase
• NCBI Official Symbol: LTC4S
• NCBI Protein Information: leukotriene C4 synthase; LTC4 synthase
• UniProt Protein Name: Leukotriene C4 synthase
• Protein Family: Leukotriene C4 synthase
• UniProt Gene Name: LTC4S
• UniProt Synonym Gene Names: LTC4 synthase
• UniProt Entry Name: LTC4S_HUMAN

NCBI Description

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family includes a number of human proteins, several of which are involved the production of leukotrienes. This gene encodes an enzyme that catalyzes the first step in the biosynthesis of cysteinyl leukotrienes, potent biological compounds derived from arachidonic acid. Leukotrienes have been implicated as mediators of anaphylaxis and inflammatory conditions such as human bronchial asthma. This protein localizes to the nuclear envelope and adjacent endoplasmic reticulum. [provided by RefSeq, Jul 2008]

Uniprot Description

LTC4S: Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4. Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency). LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly. Belongs to the MAPEG family.

Protein type: Endoplasmic reticulum; Lyase; Lipid Metabolism - arachidonic acid; EC 4.4.1.20; Membrane protein, integral; Membrane protein, multi-pass; Nuclear envelope

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: nuclear outer membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane; nuclear envelope

Molecular Function: protein binding; glutathione transferase activity; leukotriene-C4 synthase activity; enzyme activator activity; glutathione peroxidase activity; lipid binding

Biological Process: leukotriene biosynthetic process; positive regulation of catalytic activity; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Leukotriene C4 Synthase Deficiency