Leukotriene A4 Hydrolase / LTA4H Recombinant Protein | LTA4H recombinant protein

Recombinant Mouse Leukotriene A4 Hydrolase / LTA4H Protein (His tag)

Cat. Num. AAA2547067

• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: Leukotriene A4 Hydrolase / LTA4H; Recombinant Mouse Leukotriene A4 Hydrolase / LTA4H Protein (His tag); Lta4h; LTA4H recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0
• Sequence Length: 611

• Application Notes: The recombinant mouse LTA4H consists of 621 amino acids and has a calculated molecular mass of 70.4 kDa. It migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met 1
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for LTA4H recombinant protein

Background: Mouse leukotriene A-4 hydrolase, also known as LTA-4 hydrolase, Leukotriene A (4) hydrolase, LTA4H and LTA4, is cytoplasm protein which belongs to the peptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs, arachidonic acid was converted into the unstable intermediate epoxide LTA4, which may in turn be conjugated with glutathione to form the spasmogenic LTC4, or hydrolyzed into the proinflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium, and also modulates immune responses. As a bifunctional zinc metalloenzyme, LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.

Description: A DNA sequence encoding the mouse LTA4H (NP_032543.2) (Met 1-Asp 611) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 116734870
• NCBI GeneID: 16993
• NCBI Accession #: NP_032543.2
• NCBI GenBank Nucleotide #: NM_008517.2
• UniProt Accession #: P24527; Q3UY71; Q8VDR8
• Molecular Weight: 69,051 Da
• NCBI Official Full Name: leukotriene A-4 hydrolase isoform 1
• NCBI Official Synonym Full Names: leukotriene A4 hydrolase
• NCBI Official Symbol: Lta4h
• NCBI Protein Information: leukotriene A-4 hydrolase
• UniProt Protein Name: Leukotriene A-4 hydrolase
• Protein Family: Leukotriene A-4 hydrolase
• UniProt Gene Name: Lta4h
• UniProt Synonym Gene Names: LTA-4 hydrolase

NCBI Description

The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

LTA4H: Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. Belongs to the peptidase M1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.3.2.6; Hydrolase; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 10|10 C2

Cellular Component: cytoplasm; nucleoplasm; nucleus; plasma membrane

Molecular Function: aminopeptidase activity; epoxide hydrolase activity; leukotriene-A4 hydrolase activity; peptide binding; protein binding; zinc ion binding

Biological Process: leukotriene biosynthetic process; leukotriene metabolic process; peptide catabolic process; proteolysis