Target of rapamycin complex subunit LST8 (LST8) Recombinant Protein | LST8 recombinant protein

Recombinant Saccharomyces cerevisiae Target of rapamycin complex subunit LST8 (LST8)

Cat. Num. AAA1081355

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Target of rapamycin complex subunit LST8 (LST8); Recombinant Saccharomyces cerevisiae Target of rapamycin complex subunit LST8 (LST8); LST8 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-303, full length protein
• Sequence: MSVILVSAGYDHTIRFWEALTGVCSRTIQHSDSQVNRLEITNDKKLLATAGHQNVRLYDIRTTNPNPVASFEGHRGNVTSVSFQQDNRWMVTSSEDGTIKVWDVRSPSIPRNYKHNAPVNEVVIHPNQGELISCDRDGNIRIWDLGENQCTHQLTPEDDTSLQSLSMASDGSMLAAANTKGNCYVWEMPNHTDASHLKPVTKFRAHSTYITRILLSSDVKHLATCSADHTARVWSIDDDFKLETTLDGHQRWVWDCAFSADSAYLVTASSDHYVRLWDLSTREIVRQYGGHHKGAVCVALNDV
• Sequence Length: 303

• Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 398365489
• NCBI GeneID: 855726
• NCBI Accession #: NP_014392.3
• NCBI GenBank Nucleotide #: NM_001182845.3
• UniProt Accession #: P41318; D6W1H1
• Molecular Weight: 34,034 Da
• NCBI Official Full Name: TOR complex subunit LST8
• NCBI Official Symbol: LST8
• NCBI Protein Information: TOR complex subunit LST8
• UniProt Protein Name: Target of rapamycin complex subunit LST8
• UniProt Gene Name: LST8
• UniProt Synonym Gene Names: TORC subunit LST8

Uniprot Description

Essential component of both TORC1 and TORC2. TORC1 regulates multiple cellular processes to control cell growth in response to environmental signals. Nutrient limitation and environmental stress signals cause inactivation of TORC1. Active TORC1 positively controls ribosome biogenesis via control of rRNA, ribosomal protein and tRNA gene expression, and rRNA processing. TORC1 positively controls protein biosynthesis by regulation of mRNA stability, translation initiation factor activity, and high-affinity amino acid permeases that serve to provide amino acids for use by the translation machinery. TORC1 also promotes growth by sequestering a number of nutrient and general stress-responsive transcription factors in the cytoplasm. TORC1 negatively controls macroautophagy, a process to recycle surplus cytoplasmic mass under nutrient starvation conditions. LST8 is involved in the negative regulation of transcription factors GLN3 and RTG1-RTG3, limiting the synthesis of alpha-ketoglutarate, glutamate and glutamine. LST8 is required for targeting of amino acid permeases (AAPs) to the plasma membrane. TORC2 regulates cell cycle-dependent polarization of the actin-cytoskeleton, cell wall integrity, and receptor endocytosis. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway. LST8 is involved in maintenance of cell wall integrity. LST8 modulates TOR2 kinase activity.