Leucine Rich Alpha-2-Glycoprotein 1 Recombinant Protein | LRG1 recombinant protein

Recombinant Leucine Rich Alpha-2-Glycoprotein 1 (LRG1)

Cat. Num. AAA2031626

• Gene Names: LRG1; LRG; HMFT1766
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Leucine Rich Alpha-2-Glycoprotein 1; Recombinant Leucine Rich Alpha-2-Glycoprotein 1 (LRG1); LRG1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 7.9 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- CH GPTEFPSSLP ADTVHLSVEF SSLTQLPAAA LQGCPGLQEL HLSSNRLQEL SPGLLAPVPR LRVLDLTRNA LRSLPPGLFR SSAALNTLVL RENQLQEASA RWLQGLDALG YLDLAENRLR SLPARLLANL GALHTLDLGH NLLESLPEGF LRGPRRLQRL HLEGNRLQRL EAGLLAPQPF LGVLFLNDNQ LTAVAADSFR GLKQLDMLDL SNNSLSSTPP GLWAFLGRPT RDMQDGFDVS HNPWVCDKDLVDLCRWLAAN RHKMFSQN
• Sequence Length: 347

• Applicable Applications for LRG1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 33.3kDa
• Accurate Molecular Mass (KD): 33kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Ser35~Ser192
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 16418467
• NCBI GeneID: 116844
• NCBI Accession #: NP_443204.1
• NCBI GenBank Nucleotide #: NM_052972.2
• Molecular Weight: 38,178 Da
• NCBI Official Full Name: leucine-rich alpha-2-glycoprotein
• NCBI Official Synonym Full Names: leucine-rich alpha-2-glycoprotein 1
• NCBI Official Symbol: LRG1
• NCBI Official Synonym Symbols: LRG; HMFT1766
• NCBI Protein Information: leucine-rich alpha-2-glycoprotein
• UniProt Protein Name: Leucine-rich alpha-2-glycoprotein
• Protein Family: Leucine-rich alpha-2-glycoprotein
• UniProt Gene Name: LRG1
• UniProt Synonym Gene Names: LRG; LRG
• UniProt Entry Name: A2GL_HUMAN

NCBI Description

The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation (O'Donnell et al., 2002 [PubMed 12223515]).[supplied by OMIM, Mar 2008]

Uniprot Description

LRG1:

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: transforming growth factor beta receptor binding

Biological Process: positive regulation of angiogenesis; positive regulation of transforming growth factor beta receptor signaling pathway; brown fat cell differentiation; positive regulation of endothelial cell proliferation