Lysophosphatidylcholine Acyltransferase  Recombinant Protein | LPCAT1 recombinant protein

Recombinant Human Lysophosphatidylcholine Acyltransferase 

Cat. Num. AAA145687

• Gene Names: LPCAT1; AYTL2; lpcat; PFAAP3
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Lysophosphatidylcholine Acyltransferase ; Recombinant Human Lysophosphatidylcholine Acyltransferase ; LPCAT1 Human; Lysophosphatidylcholine Acyltransferase Human Recombinant; AYTL2; lpcat; PFAAP3; Lysophosphatidylcholine acyltransferase 1; LPC acyltransferase 1; LPCAT-1; LysoPC acyltransferase 1; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT; Acyltransferase-like 2; Phosphonoformate immuno-associated protein 3; LPCAT1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The LPCAT1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1M Urea and 10% glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSSAEKEPE QPPALWRKVV DFLLKAIMRT MWFAGGFHRV AVKGRQALPT EAAILTLAPH SSYFDAIPVT MTMSSIVMKA ESRDIPIWGT LIQYIRPVFV SRSDQDSRRK TVEEIKRRAQ SNGKWPQIMI FPEGTCTNRT CLITFKPGAF IPGAPVQPVV LRYPNKLDTI TWTWQGPGAL EILWLTLCQF HNQVEIEFLP VYSPSEEEKR NPALYASNVR RVMAEALGVS VTDYTFEDCQ LALAEGQLRL PADTCLLEFA RLVRGLGLKP EKLEKDLDRY SERARMKGGE KIGIAEFAAS LEVPVSDLLE DMFSLFDESG SGEVDLRECV VALSVVCRPA RTLDTIQLAF KMYGAQEDGS VGEGDLSCIL KTALGVAELT VTDLFRAIDQ EEKGKITFAD FHRFAEMYPA FAEEYLYPDQ THFESCAETS PAPIPNGFCA DFSPENSDAG RKPVRKKLD
• Sequence Length: 534

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for LPCAT1 recombinant protein

Description: LPCAT1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 479 amino acids (79-534a.a) and having a molecular mass of 42.5kDa. LPCAT1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Lysophosphatidylcholine acyltransferase 1 (LPCAT1) is a part of the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. LPCAT1 is a key enzyme for remodeling phospholipids, including phosphatidylcholine. LPCAT1 possesses both acyltransferase and acetyltransferase activities and also mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). LPCAT1 presents a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. LPCAT1 synthesizes phosphatidylcholine in pulmonary surfactant and therefore playing an important role in respiratory physiology.

Product Categories/Family for LPCAT1 recombinant protein

Enzymes; Transferase

NCBI and Uniprot Product Information

• NCBI GI #: 33946291
• NCBI GeneID: 79888
• NCBI Accession #: NP_079106.3
• NCBI GenBank Nucleotide #: NM_024830.3
• UniProt Accession #: Q8NF37; Q1HAQ1; Q7Z4G6; Q8N3U7; Q8WUL8; Q9GZW6
• Molecular Weight: 59,151 Da
• NCBI Official Full Name: lysophosphatidylcholine acyltransferase 1
• NCBI Official Synonym Full Names: lysophosphatidylcholine acyltransferase 1
• NCBI Official Symbol: LPCAT1
• NCBI Official Synonym Symbols: AYTL2; lpcat; PFAAP3
• NCBI Protein Information: lysophosphatidylcholine acyltransferase 1; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; LPC acyltransferase 1; LPCAT-1; acetyl-CoA:lyso-PAF acetyltransferase; acetyl-CoA:lyso-platelet-activating factor acetyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase 1; acyltransferase like 2; acyltransferase-like 2; lysoPAFAT; lysoPC acyltransferase 1; phosphonoformate immuno-associated protein 3; regulated by phosphonoformate
• UniProt Protein Name: Lysophosphatidylcholine acyltransferase 1
• Protein Family: Lysophosphatidylcholine acyltransferase
• UniProt Gene Name: LPCAT1
• UniProt Synonym Gene Names: AYTL2; PFAAP3; LPC acyltransferase 1; LPCAT-1; LysoPC acyltransferase 1; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT
• UniProt Entry Name: PCAT1_HUMAN

NCBI Description

Lysophosphatidylcholine (LPC) acyltransferase (LPCAT; EC 2.3.1.23) catalyzes the conversion of LPC to phosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis (Nakanishi et al., 2006 [PubMed 16704971]).[supplied by OMIM, May 2008]

Uniprot Description

LPCAT1: Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Protein type: Acetyltransferase; EC 2.3.1.67; EC 2.3.1.23; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p15.33

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane; lipid particle

Molecular Function: 1-alkylglycerophosphocholine O-acetyltransferase activity; 1-acylglycerophosphocholine O-acyltransferase activity; 1-alkenylglycerophosphocholine O-acyltransferase activity; 1-alkylglycerophosphocholine O-acyltransferase activity; calcium ion binding

Biological Process: retina development in camera-type eye; phospholipid metabolic process; glycerophospholipid biosynthetic process; positive regulation of protein catabolic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; surfactant homeostasis; phospholipid biosynthetic process