Human Lysophosphatidic acid receptor 4 ELISA Kit | LPAR4 elisa kit

Human Lysophosphatidic acid receptor 4 ELISA Kit

Cat. Num. AAA763680

• Gene Names: LPAR4; LPA4; P2Y9; GPR23; P2RY9; P2Y5-LIKE
• Reactivity: Human
• Synonyms: Lysophosphatidic acid receptor 4; Human Lysophosphatidic acid receptor 4 ELISA Kit; LPAR4/Lysophosphatidic acid receptor 4/LPA receptor 4/LPA-4/Purinergic receptor 9/P2Y5-like receptor/G-protein coupled receptor 23/P2Y purinoceptor 9 (P2Y9); LPAR4 elisa kit

• Reactivity: Human
• Sequence Length: 370

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for LPAR4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- LPAR4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- LPAR4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LPAR4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LPAR4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 487439767
• NCBI GeneID: 2846
• NCBI Accession #: NP_001264929.1
• NCBI GenBank Nucleotide #: NM_001278000.1
• Molecular Weight: 41,895 Da
• NCBI Official Full Name: lysophosphatidic acid receptor 4
• NCBI Official Synonym Full Names: lysophosphatidic acid receptor 4
• NCBI Official Symbol: LPAR4
• NCBI Official Synonym Symbols: LPA4; P2Y9; GPR23; P2RY9; P2Y5-LIKE
• NCBI Protein Information: lysophosphatidic acid receptor 4
• UniProt Protein Name: Lysophosphatidic acid receptor 4
• Protein Family: Lysophosphatidic acid receptor
• UniProt Gene Name: LPAR4
• UniProt Synonym Gene Names: GPR23; LPA4; P2RY9; LPA receptor 4; LPA-4; P2Y9
• UniProt Entry Name: LPAR4_HUMAN

NCBI Description

This gene encodes a member of the lysophosphatidic acid receptor family. It may also be related to the P2Y receptors, a family of receptors that bind purine and pyrimidine nucleotides and are coupled to G proteins. The encoded protein may play a role in monocytic differentiation. [provided by RefSeq, Feb 2009]

Uniprot Description

LPAR4: Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Transduces a signal by increasing the intracellular calcium ions and by stimulating adenylyl cyclase activity. The rank order of potency for agonists of this receptor is 1-oleoyl- > 1-stearoyl- > 1-palmitoyl- > 1-myristoyl- > 1- alkyl- > 1-alkenyl-LPA. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xq21.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway