Mouse Lctin like oxLDL Receptor 1 ELISA Kit | LOX-1 elisa kit

Mouse Lctin like oxLDL Receptor 1 ELISA Kit

Cat. Num. AAA735091

• Gene Names: Olr1; LOX-1; Oldr1; Oldlr1
• Reactivity: Mouse
• Synonyms: Lctin like oxLDL Receptor 1; Mouse Lctin like oxLDL Receptor 1 ELISA Kit; LOX-1 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 100-2500pg/mL?
• Sensitivity: 1.0pg/mL
• Intended Uses: This LOX-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of mouse LOX-1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LOX-1 elisa kit

Principle of the Assay: LOX-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LOX-1. Standards or samples are then added to the microtiter plate wells and LOX-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LOX-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for LOX-1 are added to each well to "sandwich" the LOX-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LOX-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LOX-1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4008187
• NCBI GeneID: 140914
• Molecular Weight: 41,890 Da
• NCBI Official Full Name: lectin-like oxidized low-density lipoprotein receptor
• NCBI Official Synonym Full Names: oxidized low density lipoprotein (lectin-like) receptor 1
• NCBI Official Symbol: Olr1
• NCBI Official Synonym Symbols: LOX-1; Oldr1; Oldlr1
• NCBI Protein Information: oxidized low-density lipoprotein receptor 1; ox-LDL receptor 1; lectin-like oxLDL receptor 1; lectin-like oxidized LDL receptor 1; lectin-type oxidized LDL receptor 1; lectin-like oxidized low-density lipoprotein receptor; Lectin-like oxidized low-density lipoprotein receptor-1; oxidised low density lipoprotein (lectin-like) receptor 1
• UniProt Protein Name: Oxidized low-density lipoprotein receptor 1
• UniProt Gene Name: Olr1
• UniProt Synonym Gene Names: Lox1; Oldlr1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1
• UniProt Entry Name: OLR1_RAT

NCBI Description

receptor for oxidized low-density lipoprotein that may be involved in pathogenesis of hypertension as well as atherosclerosis [RGD, Feb 2006]

Uniprot Description

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.