Monkey Laminin 11 ELISA Kit | LN11 elisa kit

Monkey Laminin 11 ELISA Kit

Cat. Num. AAA746932

• Gene Names: LAMC1; LAMB2
• Reactivity: Monkey
• Synonyms: Laminin 11; Monkey Laminin 11 ELISA Kit; LN11 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This LN11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey LN11. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This LN11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey LN11. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LN11 elisa kit

Principle of the Assay: LN11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LN11 antibody and an LN11-HRP conjugate. The assay sample and buffer are incubated together with LN11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LN11 concentration since LN11 from samples and LN11-HRP conjugate compete for the anti-LN11 antibody binding site. Since the number of sites is limited, as more sites are occupied by LN11 from the sample, fewer sites are left to bind LN11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LN11 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 145309326
• NCBI GeneID: 3915
• NCBI Accession #: NP_002284.3
• NCBI GenBank Nucleotide #: NM_002293.3
• Molecular Weight: 177,603 Da
• NCBI Official Full Name: laminin subunit gamma-1
• NCBI Official Synonym Full Names: laminin, gamma 1 (formerly LAMB2)
• NCBI Official Symbol: LAMC1
• NCBI Official Synonym Symbols: LAMB2
• NCBI Protein Information: laminin subunit gamma-1; S-LAM gamma; laminin B2 chain; S-laminin subunit gamma; laminin-2 subunit gamma; laminin-3 subunit gamma; laminin-4 subunit gamma; laminin-6 subunit gamma; laminin-7 subunit gamma; laminin-8 subunit gamma; laminin-9 subunit gamma; laminin-10 subunit gamma; laminin-11 subunit gamma
• UniProt Protein Name: Laminin subunit gamma-1
• UniProt Gene Name: LAMC1
• UniProt Synonym Gene Names: LAMB2; S-LAM gamma
• UniProt Entry Name: LAMC1_HUMAN

NCBI Description

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. [provided by RefSeq, Aug 2011]

Uniprot Description

LAMC1: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: extracellular matrix; extracellular space; laminin-1 complex; laminin-10 complex; extracellular region; basement membrane

Molecular Function: extracellular matrix structural constituent; glycosphingolipid binding

Biological Process: extracellular matrix disassembly; axon guidance; extracellular matrix organization and biogenesis; cell migration; hemidesmosome assembly; protein complex assembly; cell adhesion; positive regulation of epithelial cell proliferation; endoderm development

Disease: Pelvic Organ Prolapse, Susceptibility To