Lipase, Endothelial (LIPG) Recombinant Protein | LIPG recombinant protein

Recombinant Lipase, Endothelial (LIPG)

Cat. Num. AAA2009640

• Gene Names: LIPG; EL; EDL; PRO719
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Lipase; Endothelial (LIPG); Recombinant Lipase; LIPG recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-AGYAGN FVKGTVGRIT GLDPAGPMFE GADIHKRLSP DDADFVDVLH TYTRSFGLSI GIQMPVGHID IYPNGGDFQP GCGLNDVLGS IAYGTITEVV KCEHERAVHL FVDSLVNQDK PSFAFQCTDS NRFKKGICLS CRKNRCNSIG YNAKKMRNKR NSKMYLKTRA GMPFRVYHYQ MKIHVFSYKN MGEIEPTFYV TLYGTNADSQ TLPLEIVERI EQNATNTFLV YTEEDLGDLL KI
• Sequence Length: 500

• Applicable Applications for LIPG recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ala175~Ile412 (Accession # Q9Y5X9) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 5174497
• NCBI GeneID: 9388
• NCBI Accession #: NP_006024.1
• NCBI GenBank Nucleotide #: NM_006033.2
• UniProt Accession #: Q9Y5X9; Q6P9C8; Q6UW82; B0LPG6
• Molecular Weight: 30.5kDa
• NCBI Official Full Name: endothelial lipase
• NCBI Official Synonym Full Names: lipase, endothelial
• NCBI Official Symbol: LIPG
• NCBI Official Synonym Symbols: EL; EDL; PRO719
• NCBI Protein Information: endothelial lipase; lipoprotein lipase H; endothelial cell-derived lipase
• UniProt Protein Name: Endothelial lipase
• Protein Family: Endothelial lipase
• UniProt Gene Name: LIPG
• UniProt Synonym Gene Names: EDL; EL
• UniProt Entry Name: LIPE_HUMAN

NCBI Description

The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008]

Uniprot Description

LIPG: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Lipid Metabolism - glycerolipid; EC 3.1.1.3; Phospholipase; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: Golgi apparatus; extracellular space; cell surface; early endosome

Molecular Function: heparin binding; lipoprotein lipase activity; phospholipase A1 activity; phospholipase activity

Biological Process: cell proliferation; cholesterol homeostasis; reverse cholesterol transport; phospholipid catabolic process; regulation of lipoprotein metabolic process; lipid metabolic process; positive regulation of cholesterol transport; response to nutrient; phospholipid homeostasis