LIN7B recombinant protein

LIN7B, 1-207aa, Human, His tag, E Coli

Cat. Num. AAA204996

• Gene Names: LIN7B; MALS2; VELI2; LIN-7B; MALS-2
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: LIN7B; 1-207aa; Human; His tag; E Coli; Protein lin-7 homolog B; LIN-7B; MALS-2; MALS2; VELI2; LIN7B recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAALVEP LGLERDVSRA VELLERLQRS GELPPQKLQA LQRVLQSRFC SAIREVYEQL YDTLDITGSA EIRAHATAKA TVAAFTASEG HAHPRVVELP KTDEGLGFNI MGGKEQNSPI YISRVIPGGV ADRHGGLKRG DQLLSVNGVS VEGEQHEKAV ELLKAAQGSV KLVVRYTPRV LEEMEARFEK MRSARRRQQH QSYSSLESRG
• Sequence Length: 207

• Applicable Applications for LIN7B recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for LIN7B recombinant protein

LIN7B, also known as VELI2, belongs to the Velis family. Velis are a family of small synaptic proteins that interact with other proteins at the post-synaptic density (PSD) of neuronal synapses. Velis contain the PDZ motif involved in recruiting cell adhesion molecules, receptors, and channels. Lin7B is ubiquitously expressed with high expression in brain, liver, and testis. It localized at the synaptic junctions in neurons. Lin7B bind to CASK, a neurexin-binding protein highly concentrated in synapses, and Mint1, a binding partner with a vesicle trafficking protein. Recombinant human LIN7B protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for LIN7B recombinant protein

Signal Transduction

References

Okamoto M., et al. (1997) J Biol Chem. 272:31459-31464-293; Irie M., et al. (1999) Oncogene. 18:2811-2817.;

NCBI and Uniprot Product Information

• NCBI GI #: 11545920
• NCBI GeneID: 64130
• NCBI Accession #: NP_071448
• NCBI GenBank Nucleotide #: NM_022165.2
• UniProt Accession #: Q9HAP6
• Molecular Weight: 25.3 kDa (230aa) confirmed by MALDI-TOF
• NCBI Official Full Name: protein lin-7 homolog B isoform 1
• NCBI Official Synonym Full Names: lin-7 homolog B, crumbs cell polarity complex component
• NCBI Official Symbol: LIN7B
• NCBI Official Synonym Symbols: MALS2; VELI2; LIN-7B; MALS-2
• NCBI Protein Information: protein lin-7 homolog B
• UniProt Protein Name: Protein lin-7 homolog B
• Protein Family: Protein
• UniProt Gene Name: LIN7B
• UniProt Synonym Gene Names: MALS2; VELI2; Lin-7B; hLin7B; MALS-2; Veli-2; hVeli2

Uniprot Description

Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. May increase the amplitude of ASIC3 acid-evoked currents by stabilizing the channel at the cell surface ().