Lin7A recombinant protein

Lin7A, 1-233aa, Human, His tag, E Coli

Cat. Num. AAA204234

• Gene Names: LIN7A; LIN7; MALS1; VELI1; LIN-7A; MALS-1; TIP-33
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Lin7A; 1-233aa; Human; His tag; E Coli; Protein lin-7 homolog A; LIN-7A; LIN7; MALS-1; TIP-33; VELI1; Lin7A recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLKPSVT SAPTADMATL TVVQPLTLDR DVARAIELLE KLQESGEVPV HKLQSLKKVL QSEFCTAIRE VYQYMHETIT VNGCPEFRAR ATAKATVAAF AASEGHSHPR VVELPKTDEG LGFNVMGGKE QNSPIYISRI IPGGVAERHG GLKRGDQLLS VNGVSVEGEH HEKAVELLKA AKDSVKLVVR YTPKVLEEME ARFEKLRTAR RRQQQQLLIQ QQQQQQQQQT QQNHMS
• Sequence Length: 233

• Applicable Applications for Lin7A recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Lin7A recombinant protein

Lin7A, also as known as MALS1 or VELI1, belongs to the lin-7 family. This protein contains 1 L27 domain and 1 PDZ (DHR) domain. It plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Lin7A is expressed in brain, testis, kidney, placenta and liver. Recombinant human Lin7A protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for Lin7A recombinant protein

Signal Transduction

References

Shamshiev A., et al. (2000) Immunity 13:255-264 ; Winau F., et al. (2004) Nat. Immunol. 5:169-174

NCBI and Uniprot Product Information

• NCBI GI #: 4759306
• NCBI GeneID: 8825
• NCBI Accession #: NP_004655
• NCBI GenBank Nucleotide #: NM_004664.3
• UniProt Accession #: O14910; Q147W1; Q6LES3; Q7LDS4; A4FTY3
• Molecular Weight: 28.4 kDa (256aa) confirmed by MALDI-TOF
• NCBI Official Full Name: protein lin-7 homolog A isoform 1
• NCBI Official Synonym Full Names: lin-7 homolog A, crumbs cell polarity complex component
• NCBI Official Symbol: LIN7A
• NCBI Official Synonym Symbols: LIN7; MALS1; VELI1; LIN-7A; MALS-1; TIP-33
• NCBI Protein Information: protein lin-7 homolog A
• UniProt Protein Name: Protein lin-7 homolog A
• Protein Family: Protein
• UniProt Gene Name: LIN7A
• UniProt Synonym Gene Names: MALS1; VELI1; Lin-7A; hLin-7; MALS-1; TIP-33; Veli-1

NCBI Description

The protein encoded by this gene is involved in generating and maintaining the asymmetric distribution of channels and receptors at the cell membrane. The encoded protein also is required for the localization of some specific channels and can be part of a protein complex that couples synaptic vesicle exocytosis to cell adhesion in the brain. [provided by RefSeq, May 2016]

Uniprot Description

Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.