<
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation 293T(1.6^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-MCM3 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the IRF-1 promoter.)
Rabbit anti-Human DNA replication licensing factor MCM3 Polyclonal Antibody | anti-MCM3 antibody
Rabbit anti-human DNA replication licensing factor MCM3 polyclonal Antibody(MCM3)
Gene Names
MCM3; HCC5; P1.h; RLFB; P1-MCM3
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
DNA replication licensing factor MCM3; Polyclonal Antibody; Rabbit anti-human DNA replication licensing factor MCM3 polyclonal Antibody(MCM3); DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102; MCM3; anti-MCM3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
818
Applicable Applications for anti-MCM3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)
Application Notes
Recommended dilution: WB:1:200-1000
IHC:1:20-1:200
IHC:1:20-1:200
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human DNA replication licensing factor MCM3 protein (1-295aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-9849 / sc-9850
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation 293T(1.6^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-MCM3 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the IRF-1 promoter.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation 293T(1.6^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-MCM3 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the IRF-1 promoter.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human ovarian cancer using AAA18870 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human ovarian cancer using AAA18870 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA18870 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA18870 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver using AAA18870 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver using AAA18870 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human tonsil using AAA18870 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human tonsil using AAA18870 at dilution 1:100)
WB (Western Blot)
(All lanes: MCM3 antibody at 4 ug/mlLane 1: Hela whole cell lysateLane 2: 293T whole cell lysateLane 3: K562 whole cell lysateLane 4:HepG2 whole cell lysateLane 5:Jurkats whole cell lysateLane 6:U917 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 91,96 kDaObserved band size: 91 kDa)
WB (Western Blot)
(All lanes: MCM3 antibody at 4 ug/mlLane 1: Hela whole cell lysateLane 2: 293T whole cell lysateLane 3: K562 whole cell lysateLane 4:HepG2 whole cell lysateLane 5:Jurkats whole cell lysateLane 6:U917 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 91,96 kDaObserved band size: 91 kDa)
Related Product Information for anti-MCM3 antibody
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
95,908 Da
NCBI Official Full Name
DNA replication licensing factor MCM3 isoform 2
NCBI Official Synonym Full Names
minichromosome maintenance complex component 3
NCBI Official Symbol
MCM3
NCBI Official Synonym Symbols
HCC5; P1.h; RLFB; P1-MCM3
NCBI Protein Information
DNA replication licensing factor MCM3
UniProt Protein Name
DNA replication licensing factor MCM3
UniProt Gene Name
MCM3
UniProt Entry Name
MCM3_HUMAN
Similar Products
Product Notes
The MCM3 mcm3 (Catalog #AAA18870) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human DNA replication licensing factor MCM3 polyclonal Antibody(MCM3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNA replication licensing factor MCM3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP). Recommended dilution: WB:1:200-1000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the MCM3 mcm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA replication licensing factor MCM3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
