LIN28B recombinant protein

Recombinant Human LIN28B

Cat. Num. AAA146027

• Gene Names: LIN28B; CSDD2
• Purity: Greater than 85% as determined by SDS-PAGE.
• Synonyms: LIN28B; Recombinant Human LIN28B; LIN28B Human; LIN28B Human Recombinant; Lin-28 Homolog B (C. Elegans); Protein Lin-28 Homolog B; CSDD2; Lin-28B; Lin-28.2; FLJ16517; LIN28B recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85% as determined by SDS-PAGE.
• Form/Format: The LIN28B solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 2mM DTT and 10% glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEGGAS KGGGEEPGKL PEPAEEESQV LRGTGHCKWF NVRMGFGFIS MINREGSPLD IPVDVFVHQS KLFMEGFRSL KEGEPVEFTF KKSSKGLESI RVTGPGGSPC LGSERRPKGK TLQKRKPKGD RCYNCGGLDH HAKECSLPPQ PKKCHYCQSI MHMVANCPHK NVAQPPASSQ GRQEAESQPC TSTLPREVGG GHGCTSPPFP QEARAEISER SGRSPQEASS TKSSIAPEEQ SKKGPSVQKR KKT
• Sequence Length: 250

Related Product Information for LIN28B recombinant protein

Description: LIN28B Human Recombinant produced in E Coli is a single polypeptide chain containing 273 amino acids (1-250) and having a molecular mass of 29.5 kDa. LIN28B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Lin28B is a member of the lin-28 family. LIN28 protein is a microRNA-binding protein that binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA. Lin28B acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. LIN28 is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines.

Product Categories/Family for LIN28B recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 51972214
• NCBI GeneID: 389421
• NCBI Accession #: NP_001004317.1
• NCBI GenBank Nucleotide #: NM_001004317.3
• UniProt Accession #: Q6ZN17; Q5TCM4; A1L165; B2RPN6
• Molecular Weight: 19,511 Da
• NCBI Official Full Name: protein lin-28 homolog B
• NCBI Official Synonym Full Names: lin-28 homolog B (C. elegans)
• NCBI Official Symbol: LIN28B
• NCBI Official Synonym Symbols: CSDD2
• NCBI Protein Information: protein lin-28 homolog B; Lin-28.2; lin-28B
• UniProt Protein Name: Protein lin-28 homolog B
• Protein Family: Protein
• UniProt Gene Name: LIN28B
• UniProt Synonym Gene Names: CSDD2; Lin-28B
• UniProt Entry Name: LN28B_HUMAN

NCBI Description

The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. [provided by RefSeq, Jun 2012]

Uniprot Description

LIN28B: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. Interacts with ZCCHC11/TUT4. Might be negatively regulated by the microRNA let-7b. High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta. Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues. Belongs to the lin-28 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; RNA binding

Biological Process: regulation of transcription, DNA-dependent; pre-microRNA processing; RNA 3'-end processing