Rat LGR-5 (leucine-rich repeat-containing g-protein coupled receptor 5) ELISA Kit | Lgr5 elisa kit

Rat LGR-5 (leucine-rich repeat-containing g-protein coupled receptor 5) ELISA Kit

Cat. Num. AAA7612459

• Gene Names: Lgr5; Gpr49
• Reactivity: Rat
• Synonyms: LGR-5 (leucine-rich repeat-containing g-protein coupled receptor 5); Rat LGR-5 (leucine-rich repeat-containing g-protein coupled receptor 5) ELISA Kit; Leucine-rich repeat-containing G-protein coupled receptor 5; Lgr5; Lgr5 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lgr5. No significant cross-reactivity or interference between Lgr5 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Lgr5 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 157817525
• NCBI GeneID: 299802
• NCBI Accession #: NP_001100254.1
• NCBI GenBank Nucleotide #: NM_001106784.1
• UniProt Accession #: D4AC13
• Molecular Weight: 99,958 Da
• NCBI Official Full Name: leucine-rich repeat-containing G-protein coupled receptor 5
• NCBI Official Synonym Full Names: leucine rich repeat containing G protein coupled receptor 5
• NCBI Official Symbol: Lgr5
• NCBI Official Synonym Symbols: Gpr49
• NCBI Protein Information: leucine-rich repeat-containing G-protein coupled receptor 5
• UniProt Protein Name: Leucine-rich repeat-containing G-protein coupled receptor 5
• Protein Family: Leucine-rich repeat-containing G-protein coupled receptor
• UniProt Gene Name: Lgr5
• UniProt Entry Name: LGR5_RAT

Uniprot Description

LGR5: a leucine-rich repeat-containing G-protein-coupled receptor. Receptor for R-spondins, potent stimulators of adult stem cell proliferation. A marker of cycling, long-lived and multipotent adult stem cell populations. Target gene of Wnt signaling. A Wnt receptor component that enhances Wnt signaling by soluble R-spondin proteins. Intestinal Lgr5+ stem cells give rise throughout life to proliferation of cells in crypts, which migrate along the crypt-villus axis, differentiating into five main cell types. Wnt signaling plays a crucial role in the proliferation and differentiation of these crypt epithelial cells. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: integral to plasma membrane; trans-Golgi network membrane

Molecular Function: G-protein coupled receptor activity; transmembrane receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; hair follicle development; oocyte differentiation; signal transduction; inner ear development