Identification
Placental Protein 13 Recombinant Protein | PP13 recombinant protein
Recombinant Placental Protein 13 (PP13)
Gene Names
LGALS13; PP13; GAL13; PLAC8
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
Placental Protein 13; Recombinant Placental Protein 13 (PP13); PP13 recombinant protein
Host
E.coli
Purity/Purification
> 90%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Sequence
MSSLPVPYKL PVSLSVGSCV IIKGTPIHSF INDPQLQVDF YTDMDEDSDI AFRFRVHFGN HVVMNRREFG IWMLEETTDY VPFEDGKQFE LCIYVHYNEY EIKVNGIRIY GFVHRIPPSF VKMVQVSRDISLTSVCVCN
Applicable Applications for PP13 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Met1~Asn139
Tags
N-terminal His and GST Tag
Subcellular Location
Nucleus, Cytoplasm
Traits
Freeze-dried powder
Predicted isoelectric point
6.1
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration dateunder appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration dateunder appropriate storage condition.
Identification
Identification
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted Molecular Mass: 48.5kDa
Accurate Molecular Mass: 44kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 44kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
placental protein 13
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 13
NCBI Official Symbol
LGALS13
NCBI Official Synonym Symbols
PP13; GAL13; PLAC8
NCBI Protein Information
galactoside-binding soluble lectin 13
UniProt Protein Name
Galactoside-binding soluble lectin 13
Protein Family
UniProt Gene Name
LGALS13
UniProt Synonym Gene Names
PLAC8; Gal-13; PP13; Placental protein 13
UniProt Entry Name
PP13_HUMAN
NCBI Description
Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene has lysophospholipase activity. It is composed of two identical subunits which are held together by disulfide bonds. This protein has structural similarity to several members of the beta-galactoside-binding S-type lectin family. [provided by RefSeq, Jul 2008]
Uniprot Description
LGALS13: Has lysophospholipase activity.
Protein type: Phospholipase; EC 3.1.1.5
Chromosomal Location of Human Ortholog: 19q13.1
Molecular Function: protein binding; carbohydrate binding; lysophospholipase activity
Biological Process: apoptosis; phospholipid metabolic process
Research Articles on PP13
1. Evaluation of placental protein 13 (PP13) and risk factors as markers for predicting preeclampsia.
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Product Notes
The PP13 lgals13 (Catalog #AAA2030465) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Placental Protein 13 can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the PP13 lgals13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSSLPVPYKL PVSLSVGSCV IIKGTPIHSF INDPQLQVDF YTDMDEDSDI AFRFRVHFGN HVVMNRREFG IWMLEETTDY VPFEDGKQFE LCIYVHYNEY EIKVNGIRIY GFVHRIPPSF VKMVQVSRDI SLTSVCVCN. It is sometimes possible for the material contained within the vial of "Placental Protein 13, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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