Mouse lectin, galactoside-binding, soluble, 1 ELISA Kit | LGALS1 elisa kit

Mouse Galectin-1, LGALS1 ELISA Kit

Cat. Num. AAA916526

• Gene Names: Lgals1; L14; Gal-1; Galbp; L-14.5; Lect14; AA410090; galectin-1
• Reactivity: Mouse
• Synonyms: lectin; galactoside-binding; soluble; 1; Mouse Galectin-1; LGALS1 ELISA Kit; Mouse Galectin-1 (LGALS1) ELISA kit; DKFZp686E23103; GAL1; GBP; S-Lac lectin 1; beta-galactoside-binding lectin L-14-I; galaptin; galectin 1; galectin-1; lactose-binding lectin 1; LGALS1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse LGALS1. No significant cross-reactivity or interference between Mouse LGALS1 and analogues was observed.
• Sequence Length: 135

• Assay Type: Sandwich
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for LGALS1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LGALS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LGALS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6678682
• NCBI GeneID: 16852
• NCBI Accession #: NP_032521.1
• NCBI GenBank Nucleotide #: NM_008495.2
• UniProt Accession #: P16045; P05163; P11946; P17601; Q4FZH4; Q99M27; Q9D0X0
• Molecular Weight: 14,866 Da
• NCBI Official Full Name: galectin-1
• NCBI Official Synonym Full Names: lectin, galactose binding, soluble 1
• NCBI Official Symbol: Lgals1
• NCBI Official Synonym Symbols: L14; Gal-1; Galbp; L-14.5; Lect14; AA410090; galectin-1
• NCBI Protein Information: galectin-1; galaptin; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; beta-galactoside binding protein; lectin galactoside-binding soluble 1; beta-galactoside-binding lectin L-14-I
• UniProt Protein Name: Galectin-1
• UniProt Gene Name: Lgals1
• UniProt Synonym Gene Names: Gbp; Gal-1
• UniProt Entry Name: LEG1_MOUSE

Uniprot Description

Galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Protein type: Cell adhesion

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; cell surface; cytoplasm; extracellular region; intracellular; nucleus

Molecular Function: signal transducer activity; protein binding; protein homodimerization activity; lactose binding; galactose binding; laminin binding; galactoside binding; carbohydrate binding; glycoprotein binding

Biological Process: myoblast differentiation; heterophilic cell adhesion; plasma cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; T cell costimulation; positive regulation of erythrocyte aggregation; signal transduction