3-isopropylmalate/3-methylmalate dehydrogenase (leuB) Recombinant Protein | leuB recombinant protein
Recombinant Methanocaldococcus jannaschii 3-isopropylmalate/3-methylmalate dehydrogenase (leuB)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
3-isopropylmalate/3-methylmalate dehydrogenase (leuB); Recombinant Methanocaldococcus jannaschii 3-isopropylmalate/3-methylmalate dehydrogenase (leuB); 3-isopropylmalate/3-methylmalate dehydrogenase; EC=1.1.1.85; EC=1.1.1.n5; 3-isopropylmalate dehydrogenase; 3-IPM-DH; IMDH; IPMDH; Beta-IPM dehydrogenase; D-malate dehydrogenase [decarboxylating]; EC=1.1.1.83; leuB recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-333aa; full length protein
Sequence
MHKICVIEGD GIGKEVVPAT IQVLEATGLP FEFVYAEAGD EVYKRTGKAL PEETIETALDCDAVLFGAAG ETAADVIVKL RHILDTYANI RPVKAYKGVK CLRPDIDYVI VRENTEGLYKGIEAEIDEGI TIATRVITEK ACERIFRFAF NLARERKKMG KEGKVTCAHK ANVLKLTDGLFKKIFYKVAE EYDDIKAEDY YIDAMNMYII TKPQVFDVVV TSNLFGDILS DGAAGTVGGLGLAPSANIGD EHGLFEPVHG SAPDIAGKKI ANPTATILSA VLMLRYLGEY EAADKVEKALEEVLALGLTT PDLGGNLNTF EMAEEVAKRV REE
Sequence Length
333
Species
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,330 Da
NCBI Official Full Name
multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase
NCBI Official Symbol
MJ_0720
NCBI Protein Information
multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase
UniProt Protein Name
3-isopropylmalate/3-methylmalate dehydrogenase
Protein Family
UniProt Gene Name
leuB
UniProt Synonym Gene Names
3-IPM-DH; IMDH; IPMDH
UniProt Entry Name
LEU3_METJA
Uniprot Description
Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate, which decarboxylates to 4-methyl-2-oxopentanoate (2-oxoisocaproate). Also catalyzes the oxidative decarboxylation of 3-methylmalate to 2-oxobutyrate, and that of D-malate to pyruvate. Cannot use NADP+ instead of NAD+. Cannot catalyze the oxidation of L-malate, L-tartrate, D-tartrate, DL-isocitrate, or DL-lactate.
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Product Notes
The leuB leub (Catalog #AAA1349639) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-333aa; full length protein. The amino acid sequence is listed below: MHKICVIEGD GIGKEVVPAT IQVLEATGLP FEFVYAEAGD EVYKRTGKAL PEETIETALD CDAVLFGAAG ETAADVIVKL RHILDTYANI RPVKAYKGVK CLRPDIDYVI VRENTEGLYK GIEAEIDEGI TIATRVITEK ACERIFRFAF NLARERKKMG KEGKVTCAHK ANVLKLTDGL FKKIFYKVAE EYDDIKAEDY YIDAMNMYII TKPQVFDVVV TSNLFGDILS DGAAGTVGGL GLAPSANIGD EHGLFEPVHG SAPDIAGKKI ANPTATILSA VLMLRYLGEY EAADKVEKAL EEVLALGLTT PDLGGNLNTF EMAEEVAKRV REE. It is sometimes possible for the material contained within the vial of "3-isopropylmalate/3-methylmalate dehydrogenase (leuB), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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