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Typical Testing Data/Standard Curve (for reference only)

Horse leptin ELISA Kit | LEP elisa kit

Horse Leptin, LEP ELISA Kit

Reactivity
Horse
Synonyms
leptin; Horse Leptin; LEP ELISA Kit; Horse Leptin (LEP) ELISA kit; FLJ94114; OB; OBS; leptin (murine obesity homolog) ; leptin (obesity homolog; mouse) ; obese; mouse; homolog of; obesity factor; LEP elisa kit
Ordering
For Research Use Only!
Reactivity
Horse
Specificity
This assay has high sensitivity and excellent specificity for detection of horse LEP. No significant cross-reactivity or interference between horse LEP and analogues was observed.
Sequence Length
145
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Competitive
Detection Range
3.12 pg/ml-200 pg/ml
Sensitivity
< 0.78 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LEP elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LEP. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for LEP. The competitive inhibition reaction is launched between with pre-coated LEP and LEP in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of LEP in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
15,935 Da
NCBI Official Full Name
Leptin
NCBI Official Symbol
LEP
NCBI Protein Information
leptin; obesity factor
UniProt Protein Name
Leptin
Protein Family
UniProt Gene Name
LEP
UniProt Synonym Gene Names
OB
UniProt Entry Name
LEP_HORSE

Uniprot Description

Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass

Research Articles on LEP

Similar Products

Product Notes

The Horse LEP lep (Catalog #AAA936352) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA936352 ELISA Kit recognizes Horse LEP. It is sometimes possible for the material contained within the vial of "leptin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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