Chicken leptin ELISA Kit | LEP elisa kit

Chicken Leptin, LEP ELISA Kit

Cat. Num. AAA701850

• Reactivity: Chicken
• Synonyms: leptin; Chicken Leptin; LEP ELISA Kit; LEP ELISA kit; FLJ94114; OB; OBS; leptin (murine obesity homolog) ; leptin (obesity homolog; mouse) ; obese; mouse; homolog of; obesity factor; LEP elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chicken LEP. No significant cross-reactivity or interference between Chicken LEP and analogues was observed.
• Sequence Length: 163

• Samples: Serum, plasma and tissue homogenates
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LEP elisa kit

Introduction: Leptin is a 16 kDa protein hormone that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. Leptin is one of the most important adipose derived hormones. The Ob(Lep) gene (Ob for obese, Lep for leptin) is located on chromosome 7 in humans. Leptin acts on receptors in the hypothalamus of the brain where it inhibits appetite by (1) counteracting the effects of neuropeptide Y (a potent feeding stimulant secreted by cells in the gut and in the hypothalamus); (2) counteracting the effects of anandamide (another potent feeding stimulant that binds to the same receptors as THC), and (3) promoting the synthesis of alpha-MSH, an appetite suppressant. This inhibition is long-term, in contrast to the rapid inhibition of eating by cholecystokinin (CCK) and the slower suppression of hunger between meals mediated by PYY3-36. The absence of leptin (or its receptor) leads to uncontrolled food intake and resulting obesity. Several studies have shown that fasting or following a very-low-calorie diet (VLCD) lowers leptin levels. It might be that, in the short-term, leptin is an indicator of energy balance. It might be that the dynamics of leptin due to an acute change in energy balance are related to appetite and eventually to food intake. Although this is a new hypothesis, there are already some data that support it.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to leptin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for leptin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain leptin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of leptin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 3024234
• UniProt Accession #: O42164
• Molecular Weight: 18,183 Da
• NCBI Official Full Name: Leptin
• UniProt Protein Name: Leptin
• Protein Family: Leptin
• UniProt Gene Name: LEP
• UniProt Synonym Gene Names: OB
• UniProt Entry Name: LEP_CHICK

Uniprot Description

Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass