Human Opticin (OPTC) ELISA Kit | OPTC elisa kit

Human Opticin (OPTC) ELISA Kit

Cat. Num. AAA2024422

• Gene Names: OPTC; OPT
• Reactivity: Human
• Synonyms: Opticin (OPTC); Human Opticin (OPTC) ELISA Kit; OPT; Oculoglycan; OPTC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Opticin (OPTC).; No significant cross-reactivity or interference between Opticin (OPTC) and analogues was observed.
• Sequence Length: 332

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Opticin (OPTC) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Opticin (OPTC) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OPTC elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Opticin (OPTC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Opticin (OPTC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Opticin (OPTC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Opticin (OPTC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for OPTC elisa kit

Ophthalmology & Otorhinolaryngology

NCBI and Uniprot Product Information

• NCBI GI #: 7657419
• NCBI GeneID: 26254
• NCBI Accession #: NP_055174.1
• NCBI GenBank Nucleotide #: NM_014359.3
• UniProt Accession #: Q9UBM4; Q5T2G4
• Molecular Weight: 37,261 Da
• NCBI Official Full Name: opticin
• NCBI Official Synonym Full Names: opticin
• NCBI Official Symbol: OPTC
• NCBI Official Synonym Symbols: OPT
• NCBI Protein Information: opticin
• UniProt Protein Name: Opticin
• Protein Family: Opticin
• UniProt Gene Name: OPTC
• UniProt Synonym Gene Names: OPT
• UniProt Entry Name: OPT_HUMAN

NCBI Description

Opticin belongs to class III of the small leucine-rich repeat protein (SLRP) family. Members of this family are typically associated with the extracellular matrix. Opticin is present in significant quantities in the vitreous of the eye and also localizes to the cornea, iris, ciliary body, optic nerve, choroid, retina, and fetal liver. Opticin may noncovalently bind collagen fibrils and regulate fibril morphology, spacing, and organization. The opticin gene is mapped to a region of chromosome 1 that is associated with the inherited eye diseases age-related macular degeneration (AMD) and posterior column ataxia with retinosa pigmentosa (AXPC1). [provided by RefSeq, Jul 2008]

Uniprot Description

OPTC: Binds collagen fibrils. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: proteinaceous extracellular matrix

Molecular Function: extracellular matrix structural constituent

Biological Process: negative regulation of angiogenesis