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Lotus tetragonolobus Lectin

Lotus tetragonolobus Lectin (LTL) (FITC)

Applications
ELISA, Immunofluorescence
Purity
Molecular Biology Grade
Synonyms
Lotus tetragonolobus Lectin; Lotus tetragonolobus Lectin (LTL) (FITC); Lotus tetragonolobus Lectin lectin
Ordering
For Research Use Only!
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a liquid in 10mM HEPES, 0.15M sodium chloride, pH 7.5, 0.08% sodium azide. 0.1mM Ca++. Labeled with Fluorescein.
Concentration
2 mg/mL (varies by lot)
Applicable Applications for Lotus tetragonolobus Lectin lectin
ELISA (EL), Immunofluorescence (IF), Glycobiology
Application Notes
Suitable for use in ELISA
IF: 2-20UG/mL
Glycobiology: 5-20ug/mL
Optimal dilutions should be determined by the researcher.
Other applications not tested.
Emission
515nm
Excitation
495nm
Sugar Specifity
Fucose, Arabinose
Activity
>85%
Preparation and Storage
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for Lotus tetragonolobus Lectin lectin
Lotus tetragonolobus lectin is a family of closely related glycoproteins having 2 or 4 subunits of about 28,000D. The isoelectric points differ somewhat but these isolectins probably have similar specificities toward alpha-linked L-fucose containing oligosaccharides. Although many of the binding properties of Lotus lectin are similar to those of Ulex europaeus lectin I, the binding affinities and some specificities for oligosaccharides are markedly different between these fucose-specific lectins. Fluorescein labeled Lotus Tetragonolobus Lectin is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Lotus Tetragonolobus Lectin has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.
Product Categories/Family for Lotus tetragonolobus Lectin lectin

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Product Notes

The Lotus tetragonolobus Lectin (Catalog #AAA656253) is a Lectin and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Lotus tetragonolobus Lectin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL), Immunofluorescence (IF), Glycobiology. Suitable for use in ELISA IF: 2-20UG/mL Glycobiology: 5-20ug/mL Optimal dilutions should be determined by the researcher. Other applications not tested. Researchers should empirically determine the suitability of the Lotus tetragonolobus Lectin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lotus tetragonolobus Lectin, Lectin" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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