Goat Low density lipoprotein receptor ELISA Kit | LDLR elisa kit

Goat Low density lipoprotein receptor ELISA Kit

Cat. Num. AAA741163

• Gene Names: LDLR; FH; FHC; LDLCQ2
• Reactivity: Goat
• Synonyms: Low density lipoprotein receptor; Goat Low density lipoprotein receptor ELISA Kit; LDLR elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This LDLR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat LDLR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LDLR elisa kit

Principle of the assay: LDLR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LDLR antibody and an LDLR-HRP conjugate. The assay sample and buffer are incubated together with LDLR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LDLR concentration since LDLR from samples and LDLR-HRP conjugate compete for the anti-LDLR antibody binding site. Since the number of sites is limited, as more sites are occupied by LDLR from the sample, fewer sites are left to bind LDLR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LDLR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for LDLR elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 307121
• NCBI GeneID: 3949
• UniProt Accession #: P01130; Q53ZD9; Q9UDH7; B4DJZ8; B4DR00; B4DTQ3; C0JYY8; Q16479; Q59FQ1; Q6LCH2; Q6RCM4; Q9UH51
• Molecular Weight: 95,376 Da
• NCBI Official Full Name: low density lipoprotein receptor
• NCBI Official Synonym Full Names: low density lipoprotein receptor
• NCBI Official Symbol: LDLR
• NCBI Official Synonym Symbols: FH; FHC; LDLCQ2
• NCBI Protein Information: low-density lipoprotein receptor; LDL receptor; low-density lipoprotein receptor class A domain-containing protein 3
• UniProt Protein Name: Low-density lipoprotein receptor
• Protein Family: Low-density lipoprotein receptor
• UniProt Gene Name: LDLR
• UniProt Synonym Gene Names: LDL receptor
• UniProt Entry Name: LDLR_HUMAN

NCBI Description

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

Uniprot Description

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface; Receptor, misc.

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: Golgi apparatus; cell surface; membrane; integral to plasma membrane; lysosome; early endosome; late endosome; plasma membrane; coated pit; endosome membrane; receptor complex; external side of plasma membrane

Molecular Function: very-low-density lipoprotein receptor activity; low-density lipoprotein receptor activity; protein binding; low-density lipoprotein binding; clathrin heavy chain binding; calcium ion binding; glycoprotein binding

Biological Process: cholesterol metabolic process; receptor-mediated endocytosis; phototransduction, visible light; lipoprotein catabolic process; cholesterol transport; viral reproduction; cholesterol absorption; lipoprotein metabolic process; endocytosis; cholesterol homeostasis; phospholipid transport; lipid metabolic process; retinoid metabolic process

Disease: Hypercholesterolemia, Familial