Human LDL (Low Density Lipoprotein) CLIA Kit | LDL clia kit

Human LDL (Low Density Lipoprotein) CLIA Kit

Cat. Num. AAA2531809

• Gene Names: LDLR; FH; FHC; LDLCQ2
• Reactivity: Human
• Synonyms: LDL (Low Density Lipoprotein); Human LDL (Low Density Lipoprotein) CLIA Kit; LDL clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant LDL. No significant cross-reactivity or interference between LDL and analogues was observed.
• Sequence Length: 76

• Assay Type: Sandwich
• Detection Range: 1.563-100ng/mL
• Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LDL clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of LDL concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to LDL. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LDL and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LDL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of LDL. You can calculate the concentration of LDL in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 306411097
• NCBI GeneID: 3949
• Molecular Weight: 82,255 Da
• NCBI Official Full Name: low density lipoprotein, partial
• NCBI Official Synonym Full Names: low density lipoprotein receptor
• NCBI Official Symbol: LDLR
• NCBI Official Synonym Symbols: FH; FHC; LDLCQ2
• NCBI Protein Information: low-density lipoprotein receptor
• UniProt Protein Name: Low-density lipoprotein receptor
• UniProt Gene Name: LDLR
• UniProt Synonym Gene Names: LDL receptor
• UniProt Entry Name: LDLR_HUMAN

NCBI Description

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

Uniprot Description

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: Golgi apparatus; cell surface; membrane; apical part of cell; integral to plasma membrane; basolateral plasma membrane; lysosome; early endosome; late endosome; plasma membrane; endosome membrane; coated pit; receptor complex; external side of plasma membrane

Molecular Function: low-density lipoprotein receptor activity; very-low-density lipoprotein receptor activity; protein binding; protease binding; low-density lipoprotein binding; receptor activity; calcium ion binding; clathrin heavy chain binding; glycoprotein binding

Biological Process: cholesterol metabolic process; lipoprotein catabolic process; phototransduction, visible light; receptor-mediated endocytosis; cholesterol homeostasis; cholesterol transport; viral reproduction; cholesterol absorption; endocytosis; lipoprotein metabolic process; phospholipid transport; lipid metabolic process; retinoid metabolic process

Disease: Hypercholesterolemia, Familial