Mouse lactate dehydrogenase A ELISA Kit | LDHA elisa kit

Mouse L-lactate dehydrogenase A chain, LDH-A ELISA Kit

Cat. Num. AAA928126

• Gene Names: Ldha; Ldh1; Ldhm; l7R2
• Reactivity: Mouse
• Synonyms: lactate dehydrogenase A; Mouse L-lactate dehydrogenase A chain; LDH-A ELISA Kit; Mouse L-lactate dehydrogenase A chain (LDH-A) ELISA Kit; GSD11; LDH1; LDHM; PIG19; LDH muscle subunit; lactate dehydrogenase M; proliferation-inducing gene 19; renal carcinoma antigen NY-REN-59; LDHA elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse LDH-A. No significant cross-reactivity or interference between mouse LDH-A and analogues was observed.
• Sequence Length: 332

• Samples: serum, plasma, tissue homogenates
• Assay Type: Quantitative Competitive
• Detection Range: 0.156 mU/ml -10 mU/ml
• Sensitivity: <0.039 mU/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LDHA elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LDH-A. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for LDH-A. The competitive inhibition reaction is launched between with pre-coated LDH-A and LDH-A in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of LDH-A in the samples. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6754524
• NCBI GeneID: 16828
• NCBI Accession #: NP_034829.1
• NCBI GenBank Nucleotide #: NM_010699.2
• UniProt Accession #: P06151
• Molecular Weight: 36,499 Da
• NCBI Official Full Name: L-lactate dehydrogenase A chain isoform 1
• NCBI Official Synonym Full Names: lactate dehydrogenase A
• NCBI Official Symbol: Ldha
• NCBI Official Synonym Symbols: Ldh1; Ldhm; l7R2
• NCBI Protein Information: L-lactate dehydrogenase A chain; LDH-M; M-LDH; LDH muscle subunit; lactate dehydrogenase-A; lactate dehydrogenase A4; lactate dehydrogenase 1, A chain
• UniProt Protein Name: L-lactate dehydrogenase A chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: Ldha
• UniProt Synonym Gene Names: Ldh-1; Ldh1; LDH-A; LDH-M
• UniProt Entry Name: LDHA_MOUSE

NCBI Description

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to hemolytic anemia and early postimplantation death in mice. Multiple transcript variants encoding different isoforms have been found for this gene. The mouse genome contains multiple pseudogenes of this gene. [provided by RefSeq, May 2013]

Uniprot Description

LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.27; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; Apoptosis; Oxidoreductase; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - cysteine and methionine

Cellular Component: membrane; mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; oxidoreductase activity; L-lactate dehydrogenase activity; catalytic activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process: carbohydrate metabolic process; carboxylic acid metabolic process; cellular response to extracellular stimulus; lactate biosynthetic process from pyruvate