Canine Neutrophil Gelatinase Associated Lipocalin ELISA Kit | LCN2/NGAL elisa kit

Canine Neutrophil Gelatinase Associated Lipocalin ELISA Kit

Cat. Num. AAA738437

• Gene Names: LCN2; 24p3; MSFI; NGAL
• Reactivity: Canine
• Synonyms: Neutrophil Gelatinase Associated Lipocalin; Canine Neutrophil Gelatinase Associated Lipocalin ELISA Kit; LCN2/NGAL elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This NGAL/LCN2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine NGAL/LCN2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LCN2/NGAL elisa kit

Principle of the Assay: NGAL/LCN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NGAL/LCN2 antibody and an NGAL/LCN2-HRP conjugate. The assay sample and buffer are incubated together with NGAL/LCN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NGAL/LCN2 concentration since NGAL/LCN2 from samples and NGAL/LCN2-HRP conjugate compete for the anti-NGAL/LCN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NGAL/LCN2 from the sample, fewer sites are left to bind NGAL/LCN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NGAL/LCN2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for LCN2/NGAL elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 38455402
• NCBI GeneID: 3934
• NCBI Accession #: NP_005555.2
• NCBI GenBank Nucleotide #: NM_005564.3
• Molecular Weight: 22,457 Da
• NCBI Official Full Name: neutrophil gelatinase-associated lipocalin
• NCBI Official Synonym Full Names: lipocalin 2
• NCBI Official Symbol: LCN2
• NCBI Official Synonym Symbols: 24p3; MSFI; NGAL
• NCBI Protein Information: neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; oncogene 24p3; siderocalin LCN2; migration-stimulating factor inhibitor; 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• UniProt Protein Name: Neutrophil gelatinase-associated lipocalin
• UniProt Gene Name: LCN2
• UniProt Synonym Gene Names: HNL; NGAL; NGAL
• UniProt Entry Name: NGAL_HUMAN

Uniprot Description

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein homodimerization activity; protease binding; transporter activity; iron ion binding

Biological Process: response to drug; cellular response to nutrient levels; cellular iron ion homeostasis; response to virus; positive regulation of cell projection organization and biogenesis; siderophore transport; innate immune response; ion transport; response to herbicide; transmembrane transport