Typical Testing Data/Standard Curve (for reference only)
Human Lipocalin 2 ELISA Kit | LCN2 elisa kit
Human Lipocalin 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: LCN2/NGAL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LCN2/NGAL antibody and an LCN2/NGAL-HRP conjugate. The assay sample and buffer are incubated together with LCN2/NGAL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LCN2/NGAL concentration since LCN2/NGAL from samples and LCN2/NGAL-HRP conjugate compete for the anti-LCN2/NGAL antibody binding site. Since the number of sites is limited, as more sites are occupied by LCN2/NGAL from the sample, fewer sites are left to bind LCN2/NGAL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LCN2/NGAL concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: extracellular space; extracellular region; cytosol
Molecular Function: protein homodimerization activity; protease binding; transporter activity; iron ion binding
Biological Process: response to drug; cellular response to nutrient levels; cellular iron ion homeostasis; response to virus; siderophore transport; positive regulation of cell projection organization and biogenesis; innate immune response; ion transport; response to herbicide; transmembrane transport
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Product Notes
The Human LCN2 lcn2 (Catalog #AAA728292) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA728292 ELISA Kit recognizes Human LCN2. It is sometimes possible for the material contained within the vial of "Lipocalin 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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