Human Epididymal-specific lipocalin-12 ELISA Kit | LCN12 elisa kit

Human Epididymal-specific lipocalin-12 ELISA Kit

Cat. Num. AAA763393

• Reactivity: Human
• Synonyms: Epididymal-specific lipocalin-12; Human Epididymal-specific lipocalin-12 ELISA Kit; LCN12/Epididymal-specific lipocalin-12; LCN12 elisa kit

• Reactivity: Human
• Sequence Length: 192

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 62.5-4000pg/ml
• Sensitivity: 37.5pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for LCN12 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human LCN12 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human LCN12 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LCN12 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human LCN12can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 89886464
• NCBI GeneID: 286256
• NCBI Accession #: NP_848631.2
• NCBI GenBank Nucleotide #: NM_178536.3
• Molecular Weight: 21,500 Da
• NCBI Official Full Name: epididymal-specific lipocalin-12
• NCBI Official Synonym Full Names: lipocalin 12
• NCBI Official Symbol: LCN12
• NCBI Protein Information: epididymal-specific lipocalin-12
• UniProt Protein Name: Epididymal-specific lipocalin-12
• Protein Family: Epididymal-specific lipocalin
• UniProt Gene Name: LCN12
• UniProt Entry Name: LCN12_HUMAN

NCBI Description

Members of the lipocalin family, such as LCN12, have a common structure consisting of an 8-stranded antiparallel beta-barrel that forms a cup-shaped ligand-binding pocket or calyx. Lipocalins generally bind small hydrophobic ligands and transport them to specific cells (Suzuki et al., 2004 [PubMed 15363845]).[supplied by OMIM, Aug 2009]

Uniprot Description

LCN12: Binds all-trans retinoic acid and may act as a retinoid carrier protein within the epididymis. May play a role in male fertility. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: extracellular region

Molecular Function: retinoic acid binding; transporter activity

Biological Process: lipid metabolic process; long-chain fatty acid transport; transmembrane transport