Lamp2 recombinant protein

Lamp2, 26-379aa, Mouse, His tag, Baculovirus

Cat. Num. AAA206379

• Gene Names: Lamp2; Mac3; LGP-B; CD107b; Lamp-2; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Lamp2; 26-379aa; Mouse; His tag; Baculovirus; Lysosome-associated membrane glycoprotein 2; CD107b; Lamp II; Lamp-2; Lamp-2a; Lamp-2b; Lamp-2c; LGP-B; Mac3; Lamp2 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: LIVNLTDSKG TCLYAEWEMN FTITYETTNQ TNKTITIAVP DKATHDGSSC GDDRNSAKIM IQFGFAVSWA VNFTKEASHY SIHDIVLSYN TSDSTVFPGA VAKGVHTVKN PENFKVPLDV IFKCNSVLTY NLTPVVQKYW GIHLQAFVQN GTVSKNEQVC EEDQTPTTVA PIIHTTAPST TTTLTPTSTP TPTPTPTPTV GNYSIRNGNT TCLLATMGLQ LNITEEKVPF IFNINPATTN FTGSCQPQSA QLRLNNSQIK YLDFIFAVKN EKRFYLKEVN VYMYLANGSA FNISNKNLSF WDAPLGSSYM CNKEQVLSVS RAFQINTFNL KVQPFNVTKG QYSTAQDCSA DEDNLEHHHH HH
• Sequence Length: 415

• Applicable Applications for Lamp2 recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Lamp2 recombinant protein

Lamp2, also known as lysosome-associated membrane glycoprotein 2, is a major component of lysosomal membranes. It is clinically characterized by cardiomyopathy, myopathy, and variable mental retardation. Up-regulated Lamp2 at the plasma membrane serves as an indicator of cell activation of CD8+ T cells, mast cells, monocytes, and platelets. Recombinant mouse Lamp2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Lamp2 recombinant protein

Proteases & Inhibitors

References

Eskelinen EL., et al. (2003) Trends Cell Biol. 13:137-145.; Bertini E., et al. (2005) Neuropediatrics. 36:309-313.

NCBI and Uniprot Product Information

• NCBI GI #: 63054837
• NCBI GeneID: 16784
• NCBI Accession #: NP_001017959
• NCBI GenBank Nucleotide #: NM_001017959.2
• UniProt Accession #: P17047; Q3TXG5; Q8BSG8; A2A430
• Molecular Weight: 40.2kDa (362aa) 50-70KDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 2 isoform 1
• NCBI Official Synonym Full Names: lysosomal-associated membrane protein 2
• NCBI Official Symbol: Lamp2
• NCBI Official Synonym Symbols: Mac3; LGP-B; CD107b; Lamp-2; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c
• NCBI Protein Information: lysosome-associated membrane glycoprotein 2
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 2
• Protein Family: Lysosome-associated membrane glycoprotein
• UniProt Gene Name: Lamp2
• UniProt Synonym Gene Names: Lamp-2; LAMP-2; Lysosome-associated membrane protein 2; LGP-B

Uniprot Description

Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:10972293). Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (). Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032). Required for normal degradation of the contents of autophagosomes (PubMed:10972293, PubMed:12221139). Plays a role in lysosomal protein degradation in response to starvation (PubMed:27628032). Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens ().