Mouse lysosomal-associated membrane protein 2 ELISA Kit | LAMP2 elisa kit

Mouse Lysosome-associated membrane glycoprotein 2, LAMP2 ELISA Kit

Cat. Num. AAA912810

• Gene Names: Lamp2; Mac3; LGP-B; CD107b; Lamp-2; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c
• Reactivity: Mouse
• Synonyms: lysosomal-associated membrane protein 2; Mouse Lysosome-associated membrane glycoprotein 2; LAMP2 ELISA Kit; Mouse Lysosome-associated membrane glycoprotein 2 (LAMP2) ELISA kit; CD107b; LAMPB; LGP110; lysosome-associated membrane protein 2; LAMP2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse LAMP2. No significant cross-reactivity or interference between mouse LAMP2 and analogues was observed.
• Sequence Length: 415

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: < 7.81 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LAMP2 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LAMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMP2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 63054837
• NCBI GeneID: 16784
• NCBI Accession #: NP_001017959.1
• NCBI GenBank Nucleotide #: NM_001017959.2
• UniProt Accession #: P17047; Q3TXG5; Q8BSG8; A2A430
• Molecular Weight: 45,681 Da
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 2 isoform 1
• NCBI Official Synonym Full Names: lysosomal-associated membrane protein 2
• NCBI Official Symbol: Lamp2
• NCBI Official Synonym Symbols: Mac3; LGP-B; CD107b; Lamp-2; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c
• NCBI Protein Information: lysosome-associated membrane glycoprotein 2; lysosomal membrane glycoprotein 2; CD107 antigen-like family member B; lysosomal membrane glycoprotein type B
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 2
• Protein Family: Lysosome-associated membrane glycoprotein
• UniProt Gene Name: Lamp2
• UniProt Synonym Gene Names: Lamp-2; LAMP-2; Lysosome-associated membrane protein 2; LGP-B
• UniProt Entry Name: LAMP2_MOUSE

Uniprot Description

LAMP2: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. Defects in LAMP2 are the cause of Danon disease (DAND); also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes. Belongs to the LAMP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: phagocytic vesicle membrane; membrane; lysosomal membrane; lysosome; late endosome membrane; late endosome; integral to membrane; plasma membrane; platelet dense granule membrane; endosome

Molecular Function: protein domain specific binding; enzyme binding

Biological Process: protein stabilization