Human Laminin subunit beta-1 ELISA Kit | LAMB1 elisa kit

Human Laminin subunit beta-1 ELISA Kit

Cat. Num. AAA760599

• Gene Names: LAMB1; CLM; LIS5
• Reactivity: Human
• Synonyms: Laminin subunit beta-1; Human Laminin subunit beta-1 ELISA Kit; LAMB1/CLM/Laminin-6 subunit beta/Laminin-2 subunit beta/Laminin-12 subunit beta/Laminin-10 subunit beta/Laminin-8 subunit beta/Laminin B1 chain/Laminin-1 subunit beta; LAMB1 elisa kit

• Reactivity: Human
• Sequence Length: 1786

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for LAMB1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human LAMB1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human LAMB1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LAMB1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human LAMB1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 167614504
• NCBI GeneID: 3912
• NCBI Accession #: NP_002282.2
• NCBI GenBank Nucleotide #: NM_002291.2
• Molecular Weight: 198,038 Da
• NCBI Official Full Name: laminin subunit beta-1
• NCBI Official Synonym Full Names: laminin subunit beta 1
• NCBI Official Symbol: LAMB1
• NCBI Official Synonym Symbols: CLM; LIS5
• NCBI Protein Information: laminin subunit beta-1
• UniProt Protein Name: Laminin subunit beta-1
• Protein Family: Laminin
• UniProt Gene Name: LAMB1
• UniProt Entry Name: LAMB1_HUMAN

NCBI Description

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis. [provided by RefSeq, Aug 2011]

Uniprot Description

LAMB1: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: basement membrane; extracellular matrix; extracellular region; extracellular space; laminin-1 complex; laminin-10 complex; laminin-2 complex; laminin-8 complex; perinuclear region of cytoplasm

Molecular Function: enzyme binding; extracellular matrix structural constituent; glycosphingolipid binding; integrin binding; structural molecule activity

Biological Process: axon guidance; cell adhesion; embryo implantation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; neurite development; neuronal-glial interaction involved in cerebral cortex radial glia guided migration; odontogenesis; positive regulation of cell migration; positive regulation of epithelial cell proliferation

Disease: Lissencephaly 5