Rabbit anti-Human, Rat TIP47 Polyclonal Antibody | anti-M6PRBP1 antibody

TIP47 Antibody

Cat. Num. AAA151454

• Gene Names: PLIN3; PP17; TIP47; M6PRBP1
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: TIP47 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TIP47; Polyclonal Antibody; TIP47 Antibody; PP17; M6PRBP1; Perilipin-3; 47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR-binding protein; mannose-6-phosphate receptor binding protein 1; anti-M6PRBP1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TIP47 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 434

• Applicable Applications for anti-M6PRBP1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: TIP47 antibody can be used for the detection of TIP47 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TIP47 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human TIP47.
• Buffer: TIP47 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TIP47 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TIP47 in Daudi cell lysate with TIP47 antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of TIP47 in rat liver tissue with TIP47 antibody at 10 μg/mL. )

Immunofluorescence (IF)

(Immunofluorescence of TIP47 in Rat Liver cells with TIP47 antibody at 20 μg/mL.)

Related Product Information for anti-M6PRBP1 antibody

TIP47 Antibody: Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal surface by the Ras-associated protein Rab9 GTPase, enabling it to bind more efficiently to MPR cytoplasmic domains. Recently, it was shown that TIP47 regulates the expression of Rab9, as expression of siRNA to TIP47 in transfected cells dramatically decreased the half-life of the Rab9 protein in addition to stabilizing the subcellular localization of Rab9. At least two isoforms of TIP47 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 68846601
• NCBI GeneID: 10226
• UniProt Accession #: O60664; Q53G77; Q9BS03; Q9UBD7; Q9UP92; A8K4Y9; K7EQF4
• Molecular Weight: 45,803 Da
• NCBI Official Full Name: Perilipin-3
• NCBI Official Synonym Full Names: perilipin 3
• NCBI Official Symbol: PLIN3
• NCBI Official Synonym Symbols: PP17; TIP47; M6PRBP1
• NCBI Protein Information: perilipin-3; placental protein 17; 47 kDa MPR-binding protein; cargo selection protein TIP47; tail-interacting protein, 47 kD; mannose-6-phosphate receptor-binding protein 1
• UniProt Protein Name: Perilipin-3
• UniProt Gene Name: PLIN3
• UniProt Synonym Gene Names: M6PRBP1; TIP47; 47 kDa MPR-binding protein; PP17
• UniProt Entry Name: PLIN3_HUMAN

NCBI Description

Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2009]

Uniprot Description

perilipin 3: a membrane-associated protein and a coat protein for lipid droplets (LDs). It has been described as a mannose-6-phosphate receptor (MPR) binding protein required for endosome-to-Golgi transport of MPRs, but this function has been disputed. Interacts with M6PR and IGF2R via their cytoplasmic domains. Affects hepatic lipid and glucose metabolism. Belongs to the perilipin family. 3 isoforms of the human protein are produced by alternative splicing. Isoform 2 may exist as a homodimer known as PP17C.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; membrane; cytoplasm; endosome membrane; lipid particle; endosome

Molecular Function: protein binding

Biological Process: vesicle-mediated transport